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Seven subjects participated in paired hyperglycemic (16.3 \u00b1 0.3 mmol\/l) clamp studies performed with and without insulin. Brain glucose remained constant throughout (5.3 \u00b1 0.3 \u03bcmol\/g wet wt when serum insulin = 16 \u00b1 7 pmol\/l vs. 5.5 \u00b1 0.3 \u03bcmol\/g wet wt when serum insulin = 668 \u00b1 81 pmol\/l, P = NS). Glucose concentrations in gray matter\u2013rich occipital cortex and white matter\u2013rich periventricular tissue were then simultaneously measured in clamps, where plasma glucose ranged from 4.4 to 24.5 mmol\/l and insulin was infused at 0.5 mU \u00b7 kg\u20131 \u00b7 min\u20131. The relationship between plasma and brain glucose was linear in both regions. Reversible Michaelis-Menten kinetics fit these data best, and no differences were found in the kinetic constants calculated for each region. These data support the hypothesis that the majority of cerebral glucose uptake\/metabolism is an insulin-independent process in humans.<\/jats:p>","DOI":"10.2337\/diabetes.50.10.2203","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T14:04:22Z","timestamp":1173189862000},"page":"2203-2209","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":153,"title":["The Effect of Insulin on In Vivo Cerebral Glucose Concentrations and Rates of Glucose Transport\/Metabolism in Humans"],"prefix":"10.2337","volume":"50","author":[{"given":"Elizabeth R.","family":"Seaquist","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, and the"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory S.","family":"Damberg","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, and the"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Tkac","sequence":"additional","affiliation":[{"name":"Department of Radiology, University of Minnesota Medical School, Minneapolis, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Gruetter","sequence":"additional","affiliation":[{"name":"Department of Radiology, University of Minnesota Medical School, Minneapolis, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031212285071100_R1","doi-asserted-by":"crossref","unstructured":"Hertz MM, Paulson OB, Barry DI, Christiansen JS, Svendsen PA: Insulin increases glucose transfer across the blood-brain barrier in man. 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