{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:06:54Z","timestamp":1790885214345,"version":"4.1.0"},"reference-count":0,"publisher":"American Diabetes Association","issue":"suppl_1","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,2,1]]},"abstract":"<jats:p>To determine the roles of the ATP-sensitive K+ (K(ATP)) channels in endocrine pancreas more directly, two types of genetically engineered Kir6.2 mice were developed: mice expressing a dominant-negative form of Kir6.2 specifically in beta-cells (Kir6.2G132S Tg mice) and mice lacking Kir6.2 (Kir6.2-\/- or Kir6.2 null mice). The Kir6.2G132S Tg mice show severe impairment of K(ATP) channel function only in the beta-cells, whereas Kir6.2 null mice are completely defective in K(ATP) channel function in all of the cells in which Kir6.2 is a constituent of the K(ATP) channels, because of the disruption of Kir6.2. Both types of mice show abnormal architecture of the pancreatic islets. The number of beta-cells in Kir6.2G132S Tg mice decreases markedly with age, whereas that in Kir6.2-\/- mice decreases slightly. alpha-Cells, which are normally present only in the periphery of pancreatic islets, also appear in the center of the islets in both Kir6.2G132S Tg and Kir6.2-\/- mice. Interestingly, the number of peptide YY (PYY) and glucagon-positive cells is markedly increased in Kir6.2 null mice, whereas the number of PP cells and delta-cells is not altered. Apoptotic cells are detected by the TdT-mediated dUTP nick-end labeling (TUNEL) method at a high frequency in both Kir6.2G372S Tg and Kir6.2-\/- mice compared with the respective controls. Thus, studies of Kir6.2G372S Tg and Kir6.2-\/- mice indicate that K(ATP) channels play an important role in cell survival and differentiation in the endocrine pancreas.<\/jats:p>","DOI":"10.2337\/diabetes.50.2007.s48","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"S48","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":29,"title":["Roles of ATP-sensitive K+ channels in cell survival and differentiation in the endocrine pancreas."],"prefix":"10.2337","volume":"50","author":[{"given":"T","family":"Miki","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, Chiba University Graduate School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Iwanaga","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Chiba University Graduate School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Chiba University Graduate School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Ihara","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Chiba University Graduate School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Seino","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Chiba University Graduate School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/50\/suppl_1\/S48\/533157\/s48.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/50\/suppl_1\/S48\/533157\/s48.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:09:43Z","timestamp":1667405383000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/50\/suppl_1\/S48\/11600\/Roles-of-ATP-sensitive-K-channels-in-cell-survival"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2,1]]},"references-count":0,"journal-issue":{"issue":"suppl_1","published-print":{"date-parts":[[2001,2,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diabetes.50.2007.s48","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2,1]]}}}