{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:01:57Z","timestamp":1761742917605},"reference-count":45,"publisher":"American Diabetes Association","issue":"5","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:p>This study examines, at the ultrastructural level, whether the fetal porcine endocrine pancreas (insulin, glucagon, somatostatin, and pancreatic polypeptide [PP]- and islet amyloid polypeptide [IAPP]-containing cells) develops normally after transplantation under the kidney capsule in athymic mice. We have thus used an in vivo pig-to-mouse model for the differentiation of the endocrine pancreas removed from its normal milieu. Islet-like cell clusters (ICCs) were prepared from the fetal porcine pancreas as previously described and transplanted under the renal capsule of athymic mice. At various times after transplantation, the endocrine pancreas was removed and the level of differentiation was compared with the native pancreas of the same biological age. At the ultrastructural level, several sequential steps could be identified based on the morphology and hormone content of the secretory granules of the endocrine cell examined. Applying this approach, we could demonstrate that the ontogeny of the transplanted fetal pig pancreas follows the same sequential differentiation as the native pancreas. The process seems to be under stringent control, apparently directly related to the biological age of the tissue, and independent not only of the new environment under the kidney capsule but also of the adult and xenogeneic milieu provided after transplantation to the athymic nude mouse. Therefore, all four major hormone-producing cells seem to develop normally after transplantation when compared with the development in the native pancreas. IAPP was produced by the pluripotent fetal endocrine cells as well as the adult \u03b1-, \u03b2-, and \u03b4-cell granules in the native pancreas; however, in the transplanted pancreas, IAPP expression was demonstrated only in \u03b2-cells, \u03b4-cells, and PP cells. No IAPP was found in granules of the \u03b1-cell lineage. The results suggest a sequential differentiation of all four major types of islet cells from a common pluripotent progenitor cell, which seems to be located in the pancreatic ducts. Therefore, the results presented strongly suggest that the ontogeny of the four major endocrine islet cells is determined by genetic information carried by the progenitor cells and not by the systemic or local environment.<\/jats:p>","DOI":"10.2337\/diabetes.50.5.962","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"962-971","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":18,"title":["The Transplanted Fetal Endocrine Pancreas Undergoes an Inherent Sequential Differentiation Similar to That in the Native Pancreas"],"prefix":"10.2337","volume":"50","author":[{"given":"Agneta","family":"Lukinius","sequence":"first","affiliation":[{"name":"Genetics and Pathology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olle","family":"Korsgren","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Transfusion Medicine, Rudbeck Laboratory, University Hospital, Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","reference":[{"key":"2022031207171300800_R1","doi-asserted-by":"crossref","unstructured":"Pictet R, Rall L, Phelps P, Rutter W: The neural crest and origin of the insulin-producing and other gastrointestinal hormone-producing cells. 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