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To evaluate the molecular events coupled with high glucose\u2212induced PKC translocation and to examine the role of endogenously released ANG II in myocyte PKC signaling, primary cultures of adult rat ventricular myocytes were exposed to normal (5 mmol\/l) or high (25 mmol\/l) glucose for 12\u201324 h. Western blot analysis indicated that adult rat ventricular myocytes coexpress six PKC isozymes (\u03b1, \u03b21, \u03b22, \u03b4, \u03b5, and \u03b6). Translocation of five PKC isozymes (\u03b21, \u03b22, \u03b4, \u03b5, and \u03b6) was detected in response to 25 mmol\/l glucose. Inhibition of phospholipase C with tricyclodecan-9-yl-xanthogenate blocked glucose-induced translocation of PKC-\u03b22, -\u03b4, and -\u03b6. Inhibition of tyrosine kinase with genistein blocked glucose-induced translocation of PKC-\u03b21 and -\u03b4, whereas chelation of intracellular Ca2+ with 1,2-bis(2-aminophenoxy)ethane N,N,N,\u2019N\u2032-tetraacetic acid blocked translocation of PKC-\u03b21 and -\u03b22. Enzyme-linked immunosorbent assay performed on culture media from myocytes maintained in 25 mmol\/l glucose detected a twofold increase in ANG II. Addition of an AT-1R antagonist (losartan; 100 nmol\/l) to myocyte cultures blocked translocation of PKC-\u03b21, -\u03b22, -\u03b4, and -\u03b5. Phosphorylation of troponin (Tn) I was increased in myocytes exposed to 25 mmol\/l glucose. Losartan selectively inhibited Tn I serine phosphorylation but did not affect phosphorylation at threonine residues. We concluded that 1) 25 mmol\/l glucose triggers the release of ANG II by myocytes, resulting in activation of the ANG II autocrine pathway; 2) differential translocation of myocyte PKC isozymes occurs in response to 25 mmol\/l glucose and ANG II; and 3) AT-1R\u2212dependent PKC isozymes (\u03b21, \u03b22, \u03b4, and \u03b5) target Tn I serine residues.<\/jats:p>","DOI":"10.2337\/diabetes.50.8.1918","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"1918-1926","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":78,"title":["Angiotensin II Promotes Glucose-Induced Activation of Cardiac Protein Kinase C Isozymes and Phosphorylation of Troponin I"],"prefix":"10.2337","volume":"50","author":[{"given":"Ashwani","family":"Malhotra","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Nephrology, UMDNJ-New Jersey Medical School, Newark, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barinder P.S.","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Nephrology, UMDNJ-New Jersey Medical School, Newark, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Nephrology, UMDNJ-New Jersey Medical School, Newark, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Opawumi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Nephrology, UMDNJ-New Jersey Medical School, Newark, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard G.","family":"Meggs","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Nephrology, UMDNJ-New Jersey Medical School, Newark, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031207163022000_R1","doi-asserted-by":"crossref","unstructured":"The Diabetes Control and Complications Trial Research Group: The effect of intensive therapy of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. 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