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This insulin-induced increase in FA uptake was completely blocked by phloretin, sulfo-N-succinimidylpalmitate (SSP), and wortmannin, indicating the involvement of FAT\/CD36 and the dependence on phosphatidylinositol-3 (PI-3) kinase activation. Subcellular fractionation of insulin-stimulated cardiac myocytes demonstrated a 1.5-fold increase in sarcolemmal FAT\/CD36 and a 62% decrease in intracellular FAT\/CD36 with parallel changes in subcellular distribution of GLUT4. Induction of cellular contractions upon electrostimulation at 4 Hz enhanced cellular FA uptake 1.6-fold, independent of PI-3 kinase. The addition of insulin to 4 Hz-stimulated cells further stimulated FA uptake to 2.3-fold, indicating that there are at least two functionally independent intracellular FAT\/CD36 pools, one recruited by insulin and the other mobilized by contractions. In conclusion, we have demonstrated a novel role of insulin in cardiac FA utilization. Malfunctioning of insulin-induced FAT\/CD36 translocation may be involved in the development of type 2 diabetic cardiomyopathies.<\/jats:p>","DOI":"10.2337\/diabetes.51.10.3113","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"3113-3119","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":222,"title":["Insulin Stimulates Long-Chain Fatty Acid Utilization by Rat Cardiac Myocytes Through Cellular Redistribution of FAT\/CD36"],"prefix":"10.2337","volume":"51","author":[{"given":"Joost J.F.P.","family":"Luiken","sequence":"first","affiliation":[{"name":"Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debby P.Y.","family":"Koonen","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jodil","family":"Willems","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Zorzano","sequence":"additional","affiliation":[{"name":"Department de Bioqui\u0301mica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Medical Faculty, RWTH Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvan","family":"Fischer","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Medical Faculty, RWTH Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narendra N.","family":"Tandon","sequence":"additional","affiliation":[{"name":"Thrombosis and Vascular Biology Laboratory, Otsuka America Pharmaceutical, Rockville, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ger J.","family":"van der Vusse","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arend","family":"Bonen","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan F.C.","family":"Glatz","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031216295514100_R1","doi-asserted-by":"crossref","unstructured":"Zorzano A, Sevilla L, Camps M, Becker C, Meyer J, Kammermeier H, Mun\u0303oz P, Guma\u0300 A, Testar X, Palaci\u0301n M, Blasi J, Fischer Y: Regulation of glucose transport, and glucose transporters expression and trafficking in the heart: studies in cardiac myocytes. 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