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At 4 h into the saline infusion, a 2-h hyperinsulinemic (3 mU \u00b7 min\u22121 \u00b7 kg\u22121)-euglycemic clamp was conducted (Ins group). At 4 h into the lipid infusion, a 2-h saline control (Lip group) or 2-h hyperinsulinemic-euglycemic clamp (Lip + Ins group) was conducted. Arterial blood pressure, heart rate, femoral blood flow (FBF), hindleg vascular resistance, glucose infusion rate (GIR), hindleg glucose uptake (HGU), and muscle 2-deoxyglucose uptake (R\u2032g) were measured. Capillary recruitment, as measured by metabolism of infused 1-methylxanthine (1-MX), was also assessed. When compared with either Lip or Lip + Ins, Ins had no effect on arterial blood pressure, heart rate, FBF, or vascular resistance but increased GIR, HGU, and R\u2032g of soleus, plantaris, extensor digitorum longus, and gastrocnemius red muscles and hindlimb 1-MX metabolism. GIR, HGU, and R\u2032g of soleus, plantaris, gastrocnemius red, and the combined muscles and 1-MX metabolism were less in Lip + Ins than in Ins rats. HGU correlated closely with hindleg capillary recruitment (r = 0.86, P &amp;lt; 0.001) but not total hindleg blood flow. In conclusion, acute elevation of plasma free fatty acids blocks insulin-mediated glucose uptake and capillary recruitment.<\/jats:p>","DOI":"10.2337\/diabetes.51.4.1138","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"1138-1145","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":136,"title":["Lipid Infusion Impairs Physiologic Insulin-Mediated Capillary Recruitment and Muscle Glucose Uptake In Vivo"],"prefix":"10.2337","volume":"51","author":[{"given":"Lucy H.","family":"Clerk","sequence":"first","affiliation":[{"name":"From the Department of Biochemistry, School of Medicine, University of Tasmania, Hobart, Tasmania, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Rattigan","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry, School of Medicine, University of Tasmania, Hobart, Tasmania, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Clark","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry, School of Medicine, University of Tasmania, Hobart, Tasmania, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031222593917000_R1","doi-asserted-by":"crossref","unstructured":"DeFronzo RA: Lilly lecture 1987: the triumvirate: beta-cell, muscle, liver: a collusion responsible for NIDDM. 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