{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T22:51:03Z","timestamp":1771455063398,"version":"3.50.1"},"reference-count":26,"publisher":"American Diabetes Association","issue":"6","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,6,1]]},"abstract":"<jats:p>\u03b2-cell neogenesis from ductal precursors, and possibly from other pancreatic cell types, contributes to the expansion of \u03b2-cell mass during development and after diabetogenic insults in rodents. Using a mathematical model\u2212based analysis of \u03b2-cell mass, replication, and size, we recently demonstrated that neogenesis is also quantitatively important to the expansion of \u03b2-cell mass during prolonged hyperglycemia. In the present study, we examined the morphological appearance of neogenic focal areas, duct cell replication, and \u03b2-cell cluster size distribution in male Sprague Dawley rats infused with either saline or 50% glucose (2 ml\/h) for 0, 1, 2, 3, 4, 5, or 6 days. Pancreatic tissue characterized by a high density of small duct-like structures, previously described as neogenic focal areas, were present in glucose-infused rats after 2, 3, or 4 days of infusion. The cross-sectional area of the pancreas characterized as focal tissue peaked after 3 days of infusion at 2.9 \u00b1 0.8%. In contrast to the partial pancreatectomy model of \u03b2-cell regeneration, duct cell replication was not increased before or during focal area formation. However, the replication rate of cells in the duct-like structures of the focal areas was twofold greater than in cells of the common pancreatic duct and 15- to 40-fold greater than in cells of small, medium, and large ducts. Duct-cell replication was significantly reduced in small, medium, and large ducts of glucose as compared to saline-infused rats (0.21 \u00b1 0.02 vs. 0.48 \u00b1 0.04%; P &amp;lt; 0.03). Duct-associated \u03b2-cell mass was not different in glucose- and saline-infused rats (P = 0.78), whereas the number of acinar-associated single \u03b2 -cells increased by 70% after 3 and 4 days of glucose infusion. In addition to small duct-like structures, focal areas had considerable T-cell infiltration (151 \u00b1 30 T-cells\/ mm2). There was also an increase in T-cell infiltration in acinar tissue of glucose as compared to saline-infused rats (0.43 \u00b1 0.11 vs. 0.03 \u00b1 0. 01 T-cells\/mm2; P &amp;lt; 0.0001). In conclusion, these data suggest that neogenic focal areas in these glucose-infused rats do not arise from replication and differentiation of ductal progenitor cells. Rather, acinar cell transdifferentiation into \u03b2-cells and acinar cell dedifferentiation into neogenic focal areas lead to new \u03b2-cell formation during prolonged hyperglycemia.<\/jats:p>","DOI":"10.2337\/diabetes.51.6.1834","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"1834-1841","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":120,"title":["\u03b2-Cell Neogenesis During Prolonged Hyperglycemia in Rats"],"prefix":"10.2337","volume":"51","author":[{"given":"Mark","family":"Lipsett","sequence":"first","affiliation":[{"name":"From the Diabetes Research Laboratory, School of Kinesiology, Simon Fraser University, Burnaby, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane T.","family":"Finegood","sequence":"additional","affiliation":[{"name":"From the Diabetes Research Laboratory, School of Kinesiology, Simon Fraser University, Burnaby, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","reference":[{"key":"2022031209355456200_R1","doi-asserted-by":"crossref","unstructured":"Bonner-Weir S: Perspective: postnatal pancreatic beta cell growth. 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