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Tests for pancreatic KATP channels localized to insulin secretory granules led to the following observations: fluorescent sulfonylureas that bind the pancreatic KATP channel specifically label intracellular punctate structures in cells of the endocrine pancreas. The fluorescent glibenclamides colocalize with Ins-C-GFP, a live-cell fluorescent reporter of insulin granules. Expression of either SUR1-GFP or Kir6.2-GFP fusion proteins, but not expression of GFP alone, directs GFP fluorescence to insulin secretory granules. An SUR1 antibody specifically labels insulin granules identified by anti-insulin. Two different Kir6.2 antibodies specifically label insulin secretory granules identified by anti-insulin. Immunoelectron microscopy showed Kir6.2 antibodies specifically label perimeter membrane regions of the secretory granule. Relatively little or no labeling of other structures, including the plasma membrane, was found. Our results demonstrate that the insulin secretory granule is the major site of KATP channels of the endocrine pancreas.<\/jats:p>","DOI":"10.2337\/diabetes.52.3.767","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"767-776","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":80,"title":["The Insulin Secretory Granule Is the Major Site of KATP Channels of the Endocrine Pancreas"],"prefix":"10.2337","volume":"52","author":[{"given":"Xuehui","family":"Geng","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lehong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Watkins","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Robbins","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Drain","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031215410449400_R1","doi-asserted-by":"crossref","unstructured":"Schwanstecher M, Schwanstecher C, Dickel C, Chudziak F, Moshiri A, Panten U: Location of the sulphonylurea receptor at the cytoplasmic face of the \u03b2-cell membrane. 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