{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:24:57Z","timestamp":1791987897623,"version":"4.4.0"},"reference-count":45,"publisher":"American Diabetes Association","issue":"7","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,7,1]]},"abstract":"<jats:p>Adiponectin is a 29-kDa adipocyte protein that has been linked to the insulin resistance of obesity and lipodystrophy. To better understand the regulation of adiponectin expression, we measured plasma adiponectin and adipose tissue adiponectin mRNA levels in nondiabetic subjects with varying degrees of obesity and insulin resistance. Plasma adiponectin and adiponectin mRNA levels were highly correlated with each other (r = 0.80, P &amp;lt; 0.001), and obese subjects expressed significantly lower levels of adiponectin. However, a significant sex difference in adiponectin expression was observed, especially in relatively lean subjects. When men and women with a BMI &amp;lt;30 kg\/m2 were compared, women had a twofold higher percent body fat, yet their plasma adiponectin levels were 65% higher (8.6 \u00b1 1.1 and 14.2 \u00b1 1.6 \u03bcg\/ml in men and women, respectively; P &amp;lt; 0.02). Plasma adiponectin had a strong association with insulin sensitivity index (SI) (r = 0.67, P &amp;lt; 0.0001, n = 51) that was not affected by sex, but no relation with insulin secretion. To separate the effects of obesity (BMI) from SI, subjects who were discordant for SI were matched for BMI, age, and sex. Using this approach, insulin-sensitive subjects demonstrated a twofold higher plasma level of adiponectin (5.6 \u00b1 0.6 and 11.2 \u00b1 1.1 \u03bcg\/ml in insulin-resistant and insulin-sensitive subjects, respectively; P &amp;lt; 0.0005). Adiponectin expression was not related to plasma levels of leptin or interleukin-6. However, there was a significant inverse correlation between plasma adiponectin and tumor necrosis factor (TNF)-\u03b1 mRNA expression (r = \u22120.47, P &amp;lt; 0.005), and subjects with the highest levels of adiponectin mRNA expression secreted the lowest levels of TNF-\u03b1 from their adipose tissue in vitro. Thus, adiponectin expression from adipose tissue is higher in lean subjects and women, and is associated with higher degrees of insulin sensitivity and lower TNF-\u03b1 expression.<\/jats:p>","DOI":"10.2337\/diabetes.52.7.1779","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T14:04:22Z","timestamp":1173189862000},"page":"1779-1785","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":700,"title":["Adiponectin Expression From Human Adipose Tissue"],"prefix":"10.2337","volume":"52","author":[{"given":"Philip A.","family":"Kern","sequence":"first","affiliation":[{"name":"Central Arkansas Veterans Healthcare System and the Department of Medicine, Division of Endocrinology, University of Arkansas for Medical Sciences, Little Rock, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gina B.","family":"Di Gregorio","sequence":"additional","affiliation":[{"name":"Central Arkansas Veterans Healthcare System and the Department of Medicine, Division of Endocrinology, University of Arkansas for Medical Sciences, Little Rock, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Lu","sequence":"additional","affiliation":[{"name":"Central Arkansas Veterans Healthcare System and the Department of Medicine, Division of Endocrinology, University of Arkansas for Medical Sciences, Little Rock, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Negah","family":"Rassouli","sequence":"additional","affiliation":[{"name":"Central Arkansas Veterans Healthcare System and the Department of Medicine, Division of Endocrinology, University of Arkansas for Medical Sciences, Little Rock, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gouri","family":"Ranganathan","sequence":"additional","affiliation":[{"name":"Central Arkansas Veterans Healthcare System and the Department of Medicine, Division of Endocrinology, University of Arkansas for Medical Sciences, Little Rock, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031209365131000_R1","doi-asserted-by":"crossref","unstructured":"Flegal KM, Carroll MD, Ogden CL, Johnson CL: Prevalence and trends in obesity among US adults, 1999\u20132000. 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Am J Epidemiol\u2008145:614\u2013619,1997","DOI":"10.1093\/oxfordjournals.aje.a009158"},{"key":"2022031209365131000_R5","doi-asserted-by":"crossref","unstructured":"Kahn BB, Flier JS: Obesity and insulin resistance. J Clin Invest\u2008106:473\u2013481,2000","DOI":"10.1172\/JCI10842"},{"key":"2022031209365131000_R6","doi-asserted-by":"crossref","unstructured":"Ludvik B, Nolan JJ, Baloga J, Sacks D, Olefsky J: Effect of obesity on insulin resistance in normal subjects and patients with NIDDM. Diabetes\u200844:1121\u20131125,1995","DOI":"10.2337\/diabetes.44.9.1121"},{"key":"2022031209365131000_R7","doi-asserted-by":"crossref","unstructured":"DeFronzo RA, Ferrannini E: Insulin resistance: a multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidemia, and atherosclerotic cardiovascular disease. Diabetes Care\u200814:173\u2013194,1991","DOI":"10.2337\/diacare.14.3.173"},{"key":"2022031209365131000_R8","doi-asserted-by":"crossref","unstructured":"DeFronzo RA, Jacot E, Jequier E, Maeder E, Wahren J, Felber JP: The effect of insulin on the disposal of intravenous glucose: results from indirect calorimetry and hepatic and femoral venous catheterization. Diabetes\u200830:1000\u20131007,1981","DOI":"10.2337\/diab.30.12.1000"},{"key":"2022031209365131000_R9","doi-asserted-by":"crossref","unstructured":"Flier JS: The adipocyte. Storage depot or node on the energy information superhighway?Cell\u200880:15\u201318,1995","DOI":"10.1016\/0092-8674(95)90445-X"},{"key":"2022031209365131000_R10","doi-asserted-by":"crossref","unstructured":"Flier JS: The missing link with obesity?Nature\u2008409:292\u2013293,2001","DOI":"10.1038\/35053251"},{"key":"2022031209365131000_R11","doi-asserted-by":"crossref","unstructured":"Kern PA: Potential role of TNF\u03b1 and lipoprotein lipase as candidate genes for obesity. J Nutr\u2008127:1917S\u20131922S,1997","DOI":"10.1093\/jn\/127.9.1917S"},{"key":"2022031209365131000_R12","doi-asserted-by":"crossref","unstructured":"Hotamisligil GS, Spiegelman BM: Tumor necrosis factor \u03b1: a key component of the obesity-diabetes link. Diabetes\u200843:1271\u20131278,1994","DOI":"10.2337\/diabetes.43.11.1271"},{"key":"2022031209365131000_R13","doi-asserted-by":"crossref","unstructured":"Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, Hara K, Mori Y, Ide T, Murakami K, Tsuboyama-Kasaoka N, Ezaki O, Akanuma Y, Gavrilova O, Vinson C, Reitman ML, Kagechika H, Shudo K, Yoda M, Nakano Y, Tobe K, Nagai R, Kimura S, Tomita M, Froguel P, Kadowaki T: The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med\u20087:941\u2013946,2001","DOI":"10.1038\/90984"},{"key":"2022031209365131000_R14","doi-asserted-by":"crossref","unstructured":"Fruebis J, Tsao TS, Javorschi S, Ebbets-Reed D, Erickson MR, Yen FT, Bihain BE, Lodish HF: Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice. 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