{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:24:03Z","timestamp":1762100643512},"reference-count":34,"publisher":"American Diabetes Association","issue":"8","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,8,1]]},"abstract":"<jats:p>To determine if prior exercise enhances insulin-stimulated extraction of glucose by the liver, chronically catheterized dogs were submitted to 150 min of treadmill exercise or rest. After exercise or rest, dogs received portal glucose (18 \u03bcmol \u00b7 kg\u22121 \u00b7 min\u22121), peripheral somatostatin, and basal portal glucagon infusions from t = 0 to 150 min. A peripheral glucose infusion was used to clamp arterial blood glucose at 8.3 mmol\/l. Insulin was infused into the portal vein to create either basal levels or mild hyperinsulinemia. Prior exercise did not increase whole-body glucose disposal in the presence of basal insulin (25.5 \u00b1 1.5 vs. 20.3 \u00b1 1.7 \u03bcmol \u00b7 kg\u22121 \u00b7 min\u22121), but resulted in a marked enhancement in the presence of elevated insulin (97.2 \u00b1 15.1 vs. 64.4 \u00b1 7.4 \u03bcmol \u00b7 kg\u22121 \u00b7 min\u22121). Prior exercise also increased net hepatic glucose uptake in the presence of both basal insulin (7.5 \u00b1 1.2 vs. 2.9 \u00b1 2.4 \u03bcmol \u00b7 kg\u22121 \u00b7 min\u22121) and elevated insulin (22.0 \u00b1 3.5 vs. 11.5 \u00b1 1.8 \u03bcmol \u00b7 kg\u22121 \u00b7 min\u22121). Likewise, net hepatic glucose fractional extraction was increased by prior exercise with both basal insulin (0.04 \u00b1 0.01 vs. 0.01 \u00b1 0.01 \u03bcmol \u00b7 kg\u22121 \u00b7 min\u22121) and elevated insulin (0.10 \u00b1 0.01 vs. 0.05 \u00b1 0.01). Hepatic glycogen synthesis was increased by elevated insulin, but was not enhanced by prior exercise. Although the increase in glucose extraction after exercise could be ascribed to increased insulin action, the increase in hepatic glycogen synthesis was independent of it.<\/jats:p>","DOI":"10.2337\/diabetes.52.8.1897","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"1897-1903","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":27,"title":["Interaction of Insulin and Prior Exercise in Control of Hepatic Metabolism of a Glucose Load"],"prefix":"10.2337","volume":"52","author":[{"given":"R. Richard","family":"Pencek","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Freyja","family":"James","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Brooks","family":"Lacy","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kareem","family":"Jabbour","sequence":"additional","affiliation":[{"name":"Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip E.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick T.","family":"Fueger","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Wasserman","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee"},{"name":"Diabetes Research and Training Center, Vanderbilt University School of Medicine, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","reference":[{"key":"2022031209072358200_R1","doi-asserted-by":"crossref","unstructured":"Hamilton KS, Gibbons FK, Bracy DP, Lacy DB, Cherrington AD, Wasserman DH: Effect of prior exercise on the partitioning of an intestinal glucose load between splanchnic bed and skeletal muscle. J Clin Invest\u200898:125\u2013135,1996","DOI":"10.1172\/JCI118756"},{"key":"2022031209072358200_R2","doi-asserted-by":"crossref","unstructured":"Richter EA: Glucose utilization. In Handbook of Physiology. Rowell LB, Shepherd JT, Eds. New York, Oxford University Press,1996, p.912\u2013951","DOI":"10.1002\/cphy.cp120120"},{"key":"2022031209072358200_R3","doi-asserted-by":"crossref","unstructured":"Casey A, Mann R, Banister K, Fox J, Morris PG, MacDonald IA, Greenhaff PL: Effect of carbohydrate ingestion on glycogen resynthesis in human liver and skeletal muscle, measured by 13C MRS. Am J Physiol\u2008278:E65\u2013E75,2000","DOI":"10.1152\/ajpendo.2000.278.1.E65"},{"key":"2022031209072358200_R4","doi-asserted-by":"crossref","unstructured":"Matsuhisa M, Nishizawa I, Ikeda M, Kubota M, Arai S, Kawamori R, Yamasaki Y: Prior muscular contraction enhances disposal of glucose analog in the liver and muscle. Metabolism\u200847:44\u201349,1998","DOI":"10.1016\/S0026-0495(98)90191-3"},{"key":"2022031209072358200_R5","doi-asserted-by":"crossref","unstructured":"Galassetti P, Koyama Y, Coker RH, Lacy DB, Cherrington AD, Wasserman DH: Role of a negative arterial-portal venous glucose gradient in the postexercise state. Am J Physiol\u2008277:E1038\u2013E1045,1999","DOI":"10.1152\/ajpendo.1999.277.6.E1038"},{"key":"2022031209072358200_R6","doi-asserted-by":"crossref","unstructured":"Galassetti P, Coker RH, Lacy DB, Cherrington AD, Wasserman DH: Prior exercise increases net hepatic glucose uptake during a glucose load. Am J Physiol\u2008276:E1022\u2013E1029,1999","DOI":"10.1152\/ajpendo.1999.276.6.E1022"},{"key":"2022031209072358200_R7","doi-asserted-by":"crossref","unstructured":"Goodyear LJ, Kahn BB: Exercise, glucose transport, and insulin sensitivity. Annu Rev Med\u200849:235\u2013261,1998","DOI":"10.1146\/annurev.med.49.1.235"},{"key":"2022031209072358200_R8","doi-asserted-by":"crossref","unstructured":"Zorzano A, Balon TW, Goodman MN, Ruderman NB: Glycogen depletion and increased insulin sensitivity and responsiveness in muscle after exercise. Am J Physiol\u2008251:E664\u2013E669,1986","DOI":"10.1152\/ajpendo.1986.251.6.E664"},{"key":"2022031209072358200_R9","doi-asserted-by":"crossref","unstructured":"Devlin JT, Hirshman M, Horton ED, Horton ES: Enhanced peripheral and splanchnic insulin sensitivity in NIDDM men after single bout of exercise. Diabetes\u200836:434\u2013439,1987","DOI":"10.2337\/diabetes.36.4.434"},{"key":"2022031209072358200_R10","doi-asserted-by":"crossref","unstructured":"Cartee GD, Holloszy JO: Exercise increases susceptibility of muscle glucose transport to activation by various stimuli. Am J Physiol\u2008258:E390\u2013E393,1990","DOI":"10.1152\/ajpendo.1990.258.2.E390"},{"key":"2022031209072358200_R11","doi-asserted-by":"crossref","unstructured":"Cartee G, Young D, Sleeper M, Zierath J, Wallberg-Henriksson H, Holloszy J: Prolonged increase in insulin-stimulated glucose transport in muscle after exercise. Am J Physiol\u2008256:E494\u2013E499,1989","DOI":"10.1152\/ajpendo.1989.256.4.E494"},{"key":"2022031209072358200_R12","doi-asserted-by":"crossref","unstructured":"Firth R, Bell P, Marsh H, Hansen I, Rizza R: Postprandial hyperglycemia in patients with noninsulin-dependent diabetes mellitus. J Clin Invest\u200877:1525\u20131532,1986","DOI":"10.1172\/JCI112467"},{"key":"2022031209072358200_R13","doi-asserted-by":"crossref","unstructured":"Koyama Y, Galassetti P, Coker RH, Pencek RR, Lacy DB, Davis SN, Wasserman DH: Prior exercise and the response to insulin-induced hypoglycemia in the dog. Am J Physiol\u2008282:E1128\u2013E1138,2002","DOI":"10.1152\/ajpendo.00370.2001"},{"key":"2022031209072358200_R14","doi-asserted-by":"crossref","unstructured":"Chan T, Exton J: A rapid method for the determination of glycogen content and radioactivity in small quantities of tissue or isolated hepatocytes. Anal Biochem\u200871:96\u2013105,1976","DOI":"10.1016\/0003-2697(76)90014-2"},{"key":"2022031209072358200_R15","doi-asserted-by":"crossref","unstructured":"Golden S, Wals PA, Katz J: An improved procedure for the assay of glycogen synthase and phosphorylase in rat liver homogenates. Anal Biochem\u200877:436\u2013445,1977","DOI":"10.1016\/0003-2697(77)90257-3"},{"key":"2022031209072358200_R16","doi-asserted-by":"crossref","unstructured":"Lloyd B, Burrin J, Smythe P, Alberti KG: Enzymatic fluorometric continuous-flow assays for blood glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate. Clin Chem\u200824:1724\u20131729,1978","DOI":"10.1093\/clinchem\/24.10.1724"},{"key":"2022031209072358200_R17","doi-asserted-by":"crossref","unstructured":"Myers SR, McGuinness OP, Neal DW, Cherrington AD: Intraportal glucose delivery alters the relationship between net hepatic glucose uptake and the insulin concentration. J Clin Invest\u200887:930\u2013939,1991","DOI":"10.1172\/JCI115100"},{"key":"2022031209072358200_R18","doi-asserted-by":"crossref","unstructured":"Wasserman DH, Williams PE, Lacy DB, Green DR, Cherrington AD: Importance of intrahepatic mechanisms to gluconeogenesis from alanine during prolonged exercise and recovery. Am J Physiol\u2008254:E518\u2013E525,1988","DOI":"10.1152\/ajpendo.1988.254.4.E518"},{"key":"2022031209072358200_R19","doi-asserted-by":"crossref","unstructured":"Satake S, Moore MC, Igawa K, Converse M, Farmer B, Neal DW, Cherrington AD: Direct and indirect effects of insulin on glucose uptake and storage by the liver. Diabetes\u200851:1663\u20131671,2002","DOI":"10.2337\/diabetes.51.6.1663"},{"key":"2022031209072358200_R20","doi-asserted-by":"crossref","unstructured":"Richter EA, Garetto LP, Goodman MN, Ruderman NB: Muscle glucose metabolism following exercise in the rat: increased sensitivity to insulin. J Clin Invest\u200869:785\u2013793,1982","DOI":"10.1172\/JCI110517"},{"key":"2022031209072358200_R21","doi-asserted-by":"crossref","unstructured":"Cherrington AD: Banting Lecture 1997: Control of glucose uptake and release by the liver in vivo. Diabetes\u200848:1198\u20131214,1999","DOI":"10.2337\/diabetes.48.5.1198"},{"key":"2022031209072358200_R22","doi-asserted-by":"crossref","unstructured":"Fleig WE, Enderle D, Steudter S, Nother-Fleig G, Ditschuneit H: Regulation of basal and insulin-stimulated glycogen synthesis in cultured hepatocytes: inverse relationship to glycogen content. J Biol Chem\u2008262:1155\u20131160,1987","DOI":"10.1016\/S0021-9258(19)75764-0"},{"key":"2022031209072358200_R23","doi-asserted-by":"crossref","unstructured":"Galassetti P, Reed E, Messina A, Lacy DB, Wasserman DH: Effect of prior fast duration on the appearance and disposal of an intraduodenal glucose load. Am J Physiol\u2008276:E543\u2013E552,1999","DOI":"10.1152\/ajpendo.1999.276.3.E543"},{"key":"2022031209072358200_R24","doi-asserted-by":"crossref","unstructured":"Fery F, Plat L, Balasse EO: Effect of fasting on the intracellular metabolic partition of intravenously infused glucose in humans. Am J Physiol\u2008277:E815\u2212E823,1999","DOI":"10.1152\/ajpendo.1999.277.5.E815"},{"key":"2022031209072358200_R25","doi-asserted-by":"crossref","unstructured":"Wasserman DH, Cherrington AD: Hepatic fuel metabolism during exercise: role and regulation. Am J Physiol\u2008260:E811\u2013E824,1991","DOI":"10.1152\/ajpendo.1991.260.6.E811"},{"key":"2022031209072358200_R26","doi-asserted-by":"crossref","unstructured":"Laloux M, Stalmans W, Hers HG: On the mechanism by which glucocorticoids cause the activation of glycogen synthase in mouse and rat livers. Eur J Biochem\u2008136:175\u2013181,1983","DOI":"10.1111\/j.1432-1033.1983.tb07723.x"},{"key":"2022031209072358200_R27","doi-asserted-by":"crossref","unstructured":"Vanstapel F, Bollen M, de Wulf H, Stalmans W: Induction of hepatic glycogen synthesis by glucocorticoids is not mediated by insulin. Mol Cell Endocrinol\u200827:107\u2013114,1982","DOI":"10.1016\/0303-7207(82)90067-3"},{"key":"2022031209072358200_R28","doi-asserted-by":"crossref","unstructured":"Gorostiaga EM, Czerwinski SM, Hickson RC: Acute glucocorticoid effects on glycogen utilization, O2 uptake, and endurance. J Appl Physiol\u200864:1098\u20131106,1988","DOI":"10.1152\/jappl.1988.64.3.1098"},{"key":"2022031209072358200_R29","unstructured":"Galbo H: Hormonal Adaptations to Exercise. New York, Thieme-Stratton,1983"},{"key":"2022031209072358200_R30","doi-asserted-by":"crossref","unstructured":"McLane MP, Black PR, Law WR, Raymond RM: Adenosine reversal of in vivo hepatic responsiveness to insulin. Diabetes\u200839:62\u201369,1990","DOI":"10.2337\/diabetes.39.1.62"},{"key":"2022031209072358200_R31","doi-asserted-by":"crossref","unstructured":"Pencek RR, Lacy DB, James F, Jabbour KA, Fueger PT, Williams PE, Wasserman DH: Antagonism of hepatic adenosine receptors enhances net hepatic glucose uptake during a glucose load. Diabetes abstract in press,2003","DOI":"10.1093\/jn\/134.11.3042"},{"key":"2022031209072358200_R32","doi-asserted-by":"crossref","unstructured":"Hellsten-Westing Y, Kaijser L, Ekblom B, Sjodin B: Exchange of purines in human liver and skeletal muscle with short-term exhaustive exercise. Am J Physiol\u2008266:R81\u201386,1994","DOI":"10.1152\/ajpregu.1994.266.1.R81"},{"key":"2022031209072358200_R33","doi-asserted-by":"crossref","unstructured":"Oakes ND, Cooney GJ, Camilleri S, Chisholm DJ, Kraegen EW: Mechanisms of liver and muscle insulin resistance induced by chronic high-fat feeding. Diabetes\u200846:1768\u20131774,1997","DOI":"10.2337\/diabetes.46.11.1768"},{"key":"2022031209072358200_R34","unstructured":"Moore MC, Satake S, Lautz M, Farmer B, Neal DW, Cherrington AD: The effect of nonesterified fatty acids on net hepatic glucose uptake. Diabetes under revision,2003"}],"container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/52\/8\/1897\/375695\/db0803001897.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/52\/8\/1897\/375695\/db0803001897.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:18:39Z","timestamp":1667405919000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/52\/8\/1897\/13877\/Interaction-of-Insulin-and-Prior-Exercise-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8,1]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,8,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diabetes.52.8.1897","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8,1]]}}}