{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:51:33Z","timestamp":1791532293887,"version":"4.3.4"},"reference-count":19,"publisher":"American Diabetes Association","issue":"11","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,11,1]]},"abstract":"<jats:p>Ciliary neurotrophic factor (CNTF) potently reduces appetite and body weight in rodents and humans. We studied the short- and long-term effects of CNTFAx15, a second-generation CNTF analog, in diet-induced obese C57BL\/6J mice and brown adipose tissue (BAT)-deficient obese UCP1-DTA (uncoupling protein 1\u2013diphtheria toxin A) mice. CNTFAx15 administration (0.1, 0.3, or 1.0 \u03bcg \u00b7 g\u22121 \u00b7 day\u22121 s.c.) for 3 or 7 days reduced food intake and body weight (mainly body fat mass). The effect of CNTFAx15 on food intake and body weight was more pronounced in CNTFAx15-treated diet-induced obese C57BL\/6J mice compared with pair-fed controls and was associated with suppressed expression of hypothalamic neuropeptide Y and agouti gene\u2013related protein. Moreover, CNTFAx15 increased uncoupling protein 1 mRNA expression in BAT and energy expenditure in diet-induced obese C57BL\/6J mice. Longitudinal observations revealed a sustained reduction in body weight for several days post-CNTFAx15 treatment of CNTFAx15-treated but not pair-fed mice, followed by a gradual regain in body weight over 28 days. Finally, CNTFAx15 administration improved the metabolic profile in both diet-induced obese C57BL\/6J and UCP1-DTA mice and resulted in a significantly improved glycemic response to oral glucose tolerance tests in CNTFAx15-treated UCP1-DTA compared with pair-fed mice of similar body weight. These data suggest that CNTFAx15 may act through a pathway downstream of the putative point responsible for leptin resistance in diet-induced obese C57BL\/6J and UCP1-DTA mice to alter food intake, body weight, body composition, and metabolism. CNTFAx15 has delayed and persistent effects in diet-induced obese C57BL\/6J mice, which account for a reduction in body weight over and above what would be expected based on decreased foot intake alone.<\/jats:p>","DOI":"10.2337\/diabetes.53.11.2787","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"2787-2796","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":57,"title":["Ciliary Neurotrophic FactorAx15 Alters Energy Homeostasis, Decreases Body Weight, and Improves Metabolic Control in Diet-Induced Obese and UCP1-DTA Mice"],"prefix":"10.2337","volume":"53","author":[{"given":"Susann","family":"Blu\u0308her","sequence":"first","affiliation":[{"name":"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stergios","family":"Moschos","sequence":"additional","affiliation":[{"name":"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"John","family":"Bullen","sequence":"additional","affiliation":[{"name":"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efi","family":"Kokkotou","sequence":"additional","affiliation":[{"name":"Joslin Diabetes Center, Harvard Medical School, Research Division, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleftheria","family":"Maratos-Flier","sequence":"additional","affiliation":[{"name":"Joslin Diabetes Center, Harvard Medical School, Research Division, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley J.","family":"Wiegand","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals, Tarrytown, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Sleeman","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals, Tarrytown, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos S.","family":"Mantzoros","sequence":"additional","affiliation":[{"name":"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031206453776600_R1","doi-asserted-by":"crossref","unstructured":"Amyotrohpic Lateral Sclerosis CNTF Treatment Study Group: A double-blind placebo-controlled clinical trial of subcutaneous recombinant ciliary neurotrohpic factor (rHCNTF) in amyotrophic lateral sclerosis. 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