{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:13:21Z","timestamp":1790860401048,"version":"4.1.0"},"reference-count":21,"publisher":"American Diabetes Association","issue":"6","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,6,1]]},"abstract":"<jats:p>Activation of the coagulation cascade contributes to early graft loss and intraportal thrombotic events in clinical islet transplantation. Although these complications were shown to be related to the presence of tissue factor in human islet preparations, the contribution of duct cells, which represent a major contaminant of clinical islet isolates, has not been specified so far. Herein, we used flow cytometry, immunohistochemistry, RT-PCR, and functional coagulation assays to demonstrate that duct cells exert a potent factor VII-dependent procoagulant activity related to their expression of tissue factor. Both the classical membrane-bound and the recently described soluble form of tissue factor were shown to be synthesized by duct cells. We conclude that contaminating duct cells contribute to early \u03b2-cell damage after islet transplantation through their involvement in tissue factor-mediated thrombotic and inflammatory events.<\/jats:p>","DOI":"10.2337\/diabetes.53.6.1407","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"1407-1411","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":37,"title":["Human Pancreatic Duct Cells Exert Tissue Factor-Dependent Procoagulant Activity"],"prefix":"10.2337","volume":"53","author":[{"given":"Claire","family":"Beuneu","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Immunology and Institute for Medical Immunology, Universite\u0301 Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium"},{"name":"Juvenile Diabetes Research Foundation Center for \u03b2-Cell Therapy in Europe, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Vosters","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Immunology and Institute for Medical Immunology, Universite\u0301 Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium"},{"name":"Juvenile Diabetes Research Foundation Center for \u03b2-Cell Therapy in Europe, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babak","family":"Movahedi","sequence":"additional","affiliation":[{"name":"Juvenile Diabetes Research Foundation Center for \u03b2-Cell Therapy in Europe, Brussels, Belgium"},{"name":"Diabetes Research Center, Vrije Universiteit Brussels-VUB, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Remmelink","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Universite\u0301 Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Salmon","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Universite\u0301 Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Pipeleers","sequence":"additional","affiliation":[{"name":"Juvenile Diabetes Research Foundation Center for \u03b2-Cell Therapy in Europe, Brussels, Belgium"},{"name":"Diabetes Research Center, Vrije Universiteit Brussels-VUB, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Pradier","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Immunology and Institute for Medical Immunology, Universite\u0301 Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Goldman","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Immunology and Institute for Medical Immunology, Universite\u0301 Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium"},{"name":"Juvenile Diabetes Research Foundation Center for \u03b2-Cell Therapy in Europe, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vale\u0301rie","family":"Verhasselt","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Immunology and Institute for Medical Immunology, Universite\u0301 Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium"},{"name":"Juvenile Diabetes Research Foundation Center for \u03b2-Cell Therapy in Europe, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031207403080200_R1","doi-asserted-by":"crossref","unstructured":"Ryan EA, Lakey JR, Rajotte RV, Korbutt GS, Kin T, Imes S, Rabinovitch A, Elliott JF, Bigam D, Kneteman NM, Warnock GL, Larsen I, Shapiro AM: Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol. 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