{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T11:11:52Z","timestamp":1759230712081},"reference-count":50,"publisher":"American Diabetes Association","issue":"12","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,12,1]]},"abstract":"<jats:p>The development of islet cell transplantation as a cure for diabetes is limited by the shortage of human donor organs. Moreover, currently used grafts exhibit a marginal \u03b2-cell mass with an apparently low capacity for \u03b2-cell renewal and growth. Although duct-associated nonendocrine cells have often been suggested as a potential source for \u03b2-cell production, recent work in mice has demonstrated the role of \u03b2-cells in postnatal growth of the pancreatic \u03b2-cell mass. The present study investigated whether the \u03b2-cell mass can grow in implants that are virtually devoid of nonendocrine cells. Endocrine islet cells were purified from prenatal porcine pancreases (gestation &amp;gt;110 days) and implanted under the kidney capsule of nude mice. \u03b2-Cells initially presented with signs of immaturity: small size, low insulin content, undetectable C-peptide release, and an inability to correct hyperglycemia. They exhibited a proliferative activity that was highest during posttransplant week 1 (2.6 and 5% bromodeoxyuridine [BrdU]-positive \u03b2-cells 4 and 72 h posttransplant) and then decreased over 20 weeks to rates measured in the pancreas (0.2% BrdU-positive cells). \u03b2-Cell proliferation in implants first compensated for \u03b2-cell loss during posttransplant week 1 and then increased the \u03b2-cell number fourfold between posttransplant weeks 1 and 20. Rates of \u03b1-cell proliferation were only shortly and moderately increased, which explained the shift in cellular composition of the implant (\u03b2-cell 40 vs. 90% and \u03b1-cell 40 vs. 7% at the start and posttransplant week 20, respectively). \u03b2-Cells progressively matured during the 20 weeks after transplantation, with a twofold increase in cell volume, a sixfold increase in cellular insulin content, plasma C-peptide levels of 1\u20132 ng\/ml, and an ability to correct diabetes. They became structurally organized as homogenous clusters with their secretory vesicles polarized toward fenestrated capillaries. We concluded that the immature \u03b2-cell phenotype provides grafts with a marked potential for \u03b2-cell growth and differentiation and hence may have a potential role in curing diabetes. Cells with this phenotype can be isolated from prenatal organs; their presence in postnatal organs needs to be investigated.<\/jats:p>","DOI":"10.2337\/diabetes.54.12.3387","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"3387-3394","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":26,"title":["Growth and Functional Maturation of \u03b2-Cells in Implants of Endocrine Cells Purified From Prenatal Porcine Pancreas"],"prefix":"10.2337","volume":"54","author":[{"given":"Marika","family":"Bogdani","sequence":"first","affiliation":[{"name":"Diabetes Research Center, Brussels Free University and Juvenile Diabetes Research Foundation Center for Beta Cell Therapy in Diabetes, Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krista","family":"Suenens","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Brussels Free University and Juvenile Diabetes Research Foundation Center for Beta Cell Therapy in Diabetes, Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Troels","family":"Bock","sequence":"additional","affiliation":[{"name":"Research Laboratory for Stereology and Neuroscience, H:S Bispebjerg Hospital, University Hospital of Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Pipeleers-Marichal","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Brussels Free University and Juvenile Diabetes Research Foundation Center for Beta Cell Therapy in Diabetes, Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"In\u2019t Veld","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Brussels Free University and Juvenile Diabetes Research Foundation Center for Beta Cell Therapy in Diabetes, Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Pipeleers","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Brussels Free University and Juvenile Diabetes Research Foundation Center for Beta Cell Therapy in Diabetes, Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","reference":[{"key":"2022031209131073500_R1","doi-asserted-by":"crossref","unstructured":"Lacy PE, Scharp DW: Islet transplantation in treating diabetes. 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