{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T16:04:11Z","timestamp":1774368251312,"version":"3.50.1"},"reference-count":50,"publisher":"American Diabetes Association","issue":"5","license":[{"start":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T00:00:00Z","timestamp":1630281600000},"content-version":"vor","delay-in-days":3426,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,5,1]]},"abstract":"<jats:sec>\n                  <jats:title\/>\n                  <jats:p>Evidence shows that diabetes increases the risk of developing Alzheimer\u2019s disease (AD). Many efforts have been done to elucidate the mechanisms linking diabetes and AD. To demonstrate that mitochondria may represent a functional link between both pathologies, we compared the effects of AD and sucrose-induced metabolic alterations on mouse brain mitochondrial bioenergetics and oxidative status. For this purpose, brain mitochondria were isolated from wild-type (WT), triple transgenic AD (3xTg-AD), and WT mice fed 20% sucrose-sweetened water for 7 months. Polarography, spectrophotometry, fluorimetry, high-performance liquid chromatography, and electron microscopy were used to evaluate mitochondrial function, oxidative status, and ultrastructure. Western blotting was performed to determine the AD pathogenic protein levels. Sucrose intake caused metabolic alterations like those found in type 2 diabetes. Mitochondria from 3xTg-AD and sucrose-treated WT mice presented a similar impairment of the respiratory chain and phosphorylation system, decreased capacity to accumulate calcium, ultrastructural abnormalities, and oxidative imbalance. Interestingly, sucrose-treated WT mice presented a significant increase in amyloid \u03b2 protein levels, a hallmark of AD. These results show that in mice, the metabolic alterations associated to diabetes contribute to the development of AD-like pathologic features.<\/jats:p>\n               <\/jats:sec>","DOI":"10.2337\/db11-1186","type":"journal-article","created":{"date-parts":[[2012,3,17]],"date-time":"2012-03-17T01:46:45Z","timestamp":1331948805000},"page":"1234-1242","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":130,"title":["Metabolic Alterations Induced by Sucrose Intake and Alzheimer\u2019s Disease Promote Similar Brain Mitochondrial Abnormalities"],"prefix":"10.2337","volume":"61","author":[{"given":"Cristina","family":"Carvalho","sequence":"first","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Department of Life Sciences\u2013Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal"}]},{"given":"Susana","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Department of Life Sciences\u2013Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal"}]},{"given":"S\u00f3nia C.","family":"Correia","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Department of Life Sciences\u2013Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal"}]},{"given":"Renato X.","family":"Santos","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Department of Life Sciences\u2013Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal"}]},{"given":"Maria S.","family":"Santos","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Department of Life Sciences\u2013Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal"}]},{"given":"In\u00eas","family":"Baldeiras","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Laboratory of Neurochemistry-Coimbra University Hospital and Neurology Department-Faculty of Medicine, University of Coimbra, Coimbra, Portugal"}]},{"given":"Catarina R.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Institute of Biochemistry \u2013Faculty of Medicine, University of Coimbra, Coimbra, Portugal"}]},{"given":"Paula I.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, 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