{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:29:28Z","timestamp":1726100968567},"reference-count":30,"publisher":"MJS Publishing, Medical Journals Sweden AB","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AOS"],"abstract":"<jats:p>Signal regulatory protein alpha (SIRP\u03b1) is mainly expressed by cells of myeloid origin. This membrane glycoprotein is shown to be involved in regulation of different inflammatory conditions, such as colitis and arthritis. However, SIRP\u03b1 has not been investigated in relationship to periodontitis, an inflammatory condition affecting the tooth supporting tissues. We aim to investigate if resident cells in the periodontium express SIRP\u03b1 and whether a possible expression is affected by inflammatory conditions. Primary human keratinocytes, fibroblasts, periodontal ligament cells, and osteoblasts were cultured with or without the pro-inflammatory cytokines tumor necrosis factor alpha (TNF-\u03b1) or interleukin-1-beta (IL-1\u03b2). All different periodontal cell types showed a basal mRNA expression of SIRP\u03b1. Pro-inflammatory cytokines induced a 2\u20133-fold significant increase in SIRP\u03b1 expression in both cultured human gingival fibroblasts and osteoblasts but neither in keratinocytes nor in periodontal ligament cells. Tissue sections from human gingival tissue biopsies were histochemically stained for SIRP\u03b1. Epithelial keratinocytes and gingival fibroblasts stained positive in sections from periodontally healthy as well as in sections from periodontitis. In periodontitis sections, infiltrating leukocytes stained positive for SIRP\u03b1. We highlight our finding that oral keratinocytes, gingival fibroblasts, and periodontal ligament cells do express SIRP\u03b1, as this has not been presented before. The fact that inflammatory stimulation of gingival fibroblasts increased the expression of SIRP\u03b1, while an increased expression by gingival fibroblasts in periodontitis tissue in situ could not be detected, is indeed contradictory.\u00a0<\/jats:p>","DOI":"10.2340\/aos.v83.41391","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T13:02:02Z","timestamp":1726059722000},"page":"486-492","source":"Crossref","is-referenced-by-count":0,"title":["The expression of signal regulatory protein alpha (SIRP\u03b1) in periodontal cells and tissue"],"prefix":"10.2340","volume":"83","author":[{"given":"Cecilia","family":"Koskinen Holm","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Rosendahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per-Arne","family":"Oldenborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pernilla","family":"Lundberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1183","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"75425","doi-asserted-by":"crossref","unstructured":"Kinane DF, Stathopoulou PG, Papap PN. 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