{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:02:31Z","timestamp":1784710951730,"version":"3.55.0"},"reference-count":8,"publisher":"MJS Publishing, Medical Journals Sweden AB","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIiD"],"abstract":"<jats:p>Amnion-derived biomaterials have attracted interest in dentistry because of their anti-inflammatory, anti-scarring, and wound-healing properties. Among available preparation strategies, protease-processed amnion has been proposed as a method to better preserve structural integrity and support cellular responses, with preliminary evidence suggesting accelerated wound healing. This mini review examines the biological rationale for protease-processed amnion, compares it with conventional membrane processing approaches, and evaluates its possible role in extraction socket healing, periodontal regeneration, and oroantral repair. Available evidence suggests promising preclinical performance, but clinical support in intraoral settings remains limited. Key barriers include variability in processing, sterilization, storage, regulatory oversight, and the need for well-designed clinical trials before routine dental use can be recommended.<\/jats:p>","DOI":"10.2340\/biid.v13.46528","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T08:25:09Z","timestamp":1784708709000},"page":"609-611","source":"Crossref","is-referenced-by-count":0,"title":["Protease-processed amnion: biological rationale and translational constraints"],"prefix":"10.2340","volume":"13","author":[{"given":"Raghuram","family":"Manickam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijaya Nirmala","family":"Subramani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1183","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"163289","doi-asserted-by":"crossref","unstructured":"Talaska J, Niemczyk W, Demel K, Aboud A, Dziedzic M, Kr\u00f3l J, et al. Use of amniotic membranes in dentistry \u2013 literature review. Pol. Merkur. Lek. 2025;53:115\u20139.","DOI":"10.36740\/Merkur202501116"},{"key":"163290","doi-asserted-by":"crossref","unstructured":"Gulameabasse S, Gindraux F, Catros S, Fricain JC, Fenelon M. Chorion and amnion\/chorion membranes in oral and periodontal surgery: a systematic review. J Biomed Mater Res Part B Appl Biomater. 2021;109(8):1216\u201329.","DOI":"10.1002\/jbm.b.34783"},{"key":"163291","doi-asserted-by":"crossref","unstructured":"Ingraldi AL, Audet RG, Tabor AJ. The preparation and clinical efficacy of amnion-derived membranes: a review. J Funct Biomater. 2023;14(10):531.","DOI":"10.3390\/jfb14100531"},{"key":"163292","doi-asserted-by":"crossref","unstructured":"Paulraj M, Govindan K, Velladurai S, Heamchandsaravanan A, Shanmugam K, Muthusamy, P, et al. Protease-mediated recovery of amnion from placenta and its application as a bioprocessed therapeutic biomaterial for wound-healing. J Health Sci Med Res. 2026;44(2):e20251258.","DOI":"10.31584\/jhsmr.20251258"},{"key":"163293","doi-asserted-by":"crossref","unstructured":"Farina R, Trombelli L. Wound-healing of extraction sockets. Endod Topics. 2011;25(1):16\u201343.","DOI":"10.1111\/etp.12016"},{"key":"163294","unstructured":"Cullum D, Lucas M. Minimally invasive extraction site management with dehydrated amnion\/chorion membrane (dHACM): open-socket grafting. Compend Contin Educ Dent. 2019;40(3):178\u2013183."},{"key":"163295","doi-asserted-by":"crossref","unstructured":"Singh H, Singh H. Bioactive amnion as a guided tissue regeneration (GTR) membrane for treatment of isolated gingival recession. A case report. Indian J Dent. 2013;4(2):110\u20133.","DOI":"10.1016\/j.ijd.2012.12.007"},{"key":"163296","doi-asserted-by":"crossref","unstructured":"Lakshmi S, Bharani S, Ambardar K. Repair of an oroantral communication by a human amniotic membrane: a novel technique. J Korean Assoc Oral Maxillofac Surg. 2015;41(4):194\u20137.","DOI":"10.5125\/jkaoms.2015.41.4.194"}],"container-title":["Biomaterial Investigations in Dentistry"],"original-title":[],"link":[{"URL":"https:\/\/medicaljournalssweden.se\/biid\/article\/download\/46528\/54857","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/medicaljournalssweden.se\/biid\/article\/download\/46528\/54857","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T08:25:22Z","timestamp":1784708722000},"score":1,"resource":{"primary":{"URL":"https:\/\/medicaljournalssweden.se\/biid\/article\/view\/46528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,22]]},"references-count":8,"URL":"https:\/\/doi.org\/10.2340\/biid.v13.46528","relation":{},"ISSN":["2641-5275"],"issn-type":[{"value":"2641-5275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,22]]}}}