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Currently, there is no curative treatment for this disease, hence there is a need for new therapeutic suggestions. The aim of this study was to investigate the effect of hyaluronidase (HYAL) injections after inducing hindlimb lymphedema in 36 female C57BL\/6 mice. HYAL injections were administered every second day for 14 days in three groups: (1) HYAL for 1 week followed by saline for 1 week, (2) HYAL for 2 weeks, and (3) saline injections for 2 weeks. Volume of the lymphedema limb was weekly assessed with micro-computed tomography (\u03bc-CT) scans for a total course of 6 weeks. Lymph vessel morphometry was assessed in the end of the study after staining cross-sections of the hindlimb for anti-LYVE-1 blindly. Lymphatic function was assessed by lymphoscintigraphy to assess lymphatic clearance. There was a significant reduction of the volume of lymphedema in mice treated with HYAL-7 compared with mice treated with HYAL-14 (p &lt; 0.05) and saline (p &lt; 0.05). No differences were detected in lymph vessel morphometry and the lymphoscintigraphy between groups. Short-term treatment with HYAL-7 might be a potential therapeutic suggestion for secondary lymphedema induced in mouse hindlimbs. In the future, clinical studies are needed to investigate the potential of HYAL treatment in human beings.\u00a0<\/jats:p>","DOI":"10.2340\/jphs.v58.7791","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T11:31:52Z","timestamp":1687260712000},"page":"40-47","source":"Crossref","is-referenced-by-count":3,"title":["Short term treatment of secondary lymphedema with hyaluronidase injections reduces mouse hindlimb lymphedema"],"prefix":"10.2340","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2305-7758","authenticated-orcid":false,"given":"Farima","family":"Dalaei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2775-8463","authenticated-orcid":false,"given":"Amar","family":"Bucan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4760-3549","authenticated-orcid":false,"given":"Alexander","family":"Wiinholt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8755-3446","authenticated-orcid":false,"given":"Mads Gustaf","family":"J\u00f8rgensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5716-6069","authenticated-orcid":false,"given":"Christian R\u00f8nn","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5199-9200","authenticated-orcid":false,"given":"Christina","family":"Baun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8857-4521","authenticated-orcid":false,"given":"Svend","family":"Hvidsten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3462-321X","authenticated-orcid":false,"given":"Eva Kildall","family":"Hejb\u00f8l","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7588-235X","authenticated-orcid":false,"given":"Henrik Daa","family":"Schr\u00f8der","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4903-0094","authenticated-orcid":false,"given":"Jens Ahm","family":"S\u00f8rensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1183","published-online":{"date-parts":[[2023,6,20]]},"reference":[{"key":"34114","doi-asserted-by":"crossref","unstructured":"DiSipio T, Rye S, Newman B, Hayes S. 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