{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T08:51:38Z","timestamp":1777884698090,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatr Dev Pathol"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p>\n                    Large cell calcifying Sertoli cell tumors (LCCSCT) are associated with Carney complex and Peutz-Jeghers syndrome. The mechanisms linking these 2 genetic defects to the genesis of this tumor are obscure. Studies of CYP19 (aromatase) and transforming growth factor (TGF)\u2013\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    messenger RNA (mRNA) abundance, estrogen receptor (ER), TGF\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    , and TGF\u03b2 type II receptor (R) immunochemistry were carried out in the testis of a patient with this tumor to gain information on possible mechanisms of cell tumor development. Testicular tissue of a prepubertal patient, collected at gonadectomy, was separated into 2 macroscopically distinct fractions: tumoral nodules (Tu) and extratumoral, normal-looking testicular tissue (ExTu). The patient was a 9.5-year-old boy with a 5-year history of bilateral gynecomastia (Tanner stage 4), no pubic hair, incipient genital development, and bilateral testicular nodules. Multiple pigmented lesions of the skin were present. Bilateral mammectomy and gonadectomy was performed. RNA was extracted from Tu and ExTu for semiquantitative reverse transcriptase\u2013polymerase chain reaction of CYP19 and TGF\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    . Protein expression of ER, TGF\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    , and TGF\u03b2 type II R in Tu and ExTu was detected by immunohistochemistry. Cell proliferation was estimated by Ki-67 antigen immunochemistry and apoptosis using a modified TUNEL assay. Mean expression of aromatase and TGF\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    mRNAs in Tu was 6- and 2.3-fold higher than in ExTu, respectively ( P &lt; 0.05). Tumoral cells exhibited ER staining with a predominant extra-nuclear localization. Positive staining of Sertoli cells in Tu was higher than in ExTu. TGF\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    immunostaining of the interstitial cells in Tu was higher than in ExTu. TGF\u03b2 type II R immunostaining was detected in most Sertoli and interstitial cells, but intensity in ExTu was lower than in Tu. No significant difference was detected in the proliferation index, but in Tu, the percentage of Sertoli cells in apoptosis (1.4%) was significantly lower ( P &lt; 0.01) than in ExTu (14.0%). The following hypothesis is proposed. The congenital gene defects of Carney complex or of Peutz-Jeghers syndrome might trigger a cascade of intracellular events that leads to overexpression of aromatase in Sertoli cells, favoring the development of a LCCSCT. At some point in the evolution of the disease, a mutational event might induce a higher expression of the ER. Also, TGF\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    protein expression is increased in neighboring cells. In this environment, TGF\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    might switch from tumor suppressor to oncogenic factor and along with estrogen-ER complexes, might favor tumor progression by inhibiting apoptosis.\n                  <\/jats:p>","DOI":"10.2350\/06-04-0074.1","type":"journal-article","created":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T17:06:06Z","timestamp":1152723966000},"page":"181-189","source":"Crossref","is-referenced-by-count":7,"title":["High TGF\u03b2\n                    <sub>1<\/sub>\n                    , Estrogen Receptor, and Aromatase Gene Expression in a Large Cell Calcifying Sertoli Cell Tumor (LCCSCT): Implications for the Mechanism of Oncogenesis"],"prefix":"10.1177","volume":"9","author":[{"given":"Nora","family":"Saraco","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esperanza","family":"Berensztein","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariela","family":"Sciara","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria T.G.","family":"de Davila","sequence":"additional","affiliation":[{"name":"Servicio de Anatomia Patol\u00f3gica, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Ciaccio","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"Belgorosky","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco A.","family":"Rivarola","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n, Hospital de Pediatria Garrahan, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,5,1]]},"reference":[{"key":"bibr1-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1159\/000068770"},{"key":"bibr2-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2003.1110121.x"},{"key":"bibr3-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.86.9.7903"},{"key":"bibr4-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg160"},{"key":"bibr5-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1016\/S1096-7192(03)00006-4"},{"key":"bibr6-06-04-0074.1","first-page":"365","volume":"36","author":"Wang ZJ","year":"1999","journal-title":"J Med Genet"},{"key":"bibr7-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-4924-2-28"},{"key":"bibr8-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200110000-00003"},{"key":"bibr9-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(97)80026-X"},{"key":"bibr10-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64736-0"},{"key":"bibr11-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(94)00041-A"},{"key":"bibr12-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00444-0"},{"key":"bibr13-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.17.1388"},{"key":"bibr14-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4652(200002)186:2<153::AID-JCP1016>3.0.CO;2-J"},{"key":"bibr15-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105316200"},{"key":"bibr16-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1515\/JPEM.2000.13.5.483"},{"key":"bibr17-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20000520)89:3<251::AID-IJC7>3.0.CO;2-5"},{"key":"bibr18-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-020032"},{"key":"bibr19-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.13.1497"},{"key":"bibr20-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200318899"},{"key":"bibr21-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206871"},{"key":"bibr22-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1208"},{"key":"bibr23-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-48158-8_12"},{"key":"bibr24-06-04-0074.1","first-page":"3546","volume":"63","author":"Kinoshita Y","year":"2003","journal-title":"Cancer Res"},{"key":"bibr25-06-04-0074.1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-1470"}],"container-title":["Pediatric and Developmental Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.2350\/06-04-0074.1","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.2350\/06-04-0074.1","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.2350\/06-04-0074.1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T02:34:48Z","timestamp":1777602888000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.2350\/06-04-0074.1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.2350\/06-04-0074.1"],"URL":"https:\/\/doi.org\/10.2350\/06-04-0074.1","relation":{},"ISSN":["1093-5266","1615-5742"],"issn-type":[{"value":"1093-5266","type":"print"},{"value":"1615-5742","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]}}}