{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,15]],"date-time":"2026-11-15T00:15:15Z","timestamp":1794701715297,"version":"4.9.1"},"reference-count":29,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Pediatr Dev Pathol"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>NRAS and BRAF mutations occur in congenital melanocytic nevi (CMN), but results are contradictory. Sixty-six prospectively collected CMN patients were analyzed for NRAS Q61 mutations using Sanger sequencing. Negative cases were evaluated for BRAF V600E mutation. NRAS Q61 mutations affected 51 patients (77.3%), and BRAF V600E was found in 5 (7.6%). NRAS Q61 mutation affected 29 (80.6%) of 36 giant, 16 (80.0%) of 20 large, and 5 (62.5%) of 8 medium-size CMN; BRAF mutation affected 1 (5%) of 20 large and 4 (11.4%) of 36 giant CMN. Compared to NRAS, BRAF-mutated nevi show scattered\/extensive dermal and subcutaneous nodules (100% BRAF+ vs 34.8% NRAS+) ( P = 0.002). Neurocutaneous melanocytosis (NCM) affected 16 (24.2%) of 66 patients, with NRAS Q61 mutation in 12 (75.0%), and BRAF V600E in 2 (12.5%), P = 0.009. Two patients were negative for both mutations (12.5%). In conclusion, although NRAS Q61 mutations predominate, BRAF V600E mutation also affects patients with large\/giant CMN (L\/GCMN), and with NCM, a novel finding. BRAF V600E is also associated with increased dermal\/subcutaneous nodules. These findings open the possibility of BRAF-targeted therapy in some L\/GCMN and NCM cases.<\/jats:p>","DOI":"10.2350\/14-10-1566-oa.1","type":"journal-article","created":{"date-parts":[[2014,12,9]],"date-time":"2014-12-09T13:18:37Z","timestamp":1418131117000},"page":"1-9","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":81,"title":["<i>BRAF<\/i>\n                    Mutations are Also Associated with Neurocutaneous Melanocytosis and Large\/Giant Congenital Melanocytic Nevi"],"prefix":"10.1177","volume":"18","author":[{"given":"Cl\u00e1udia M.","family":"Salgado","sequence":"first","affiliation":[{"name":"Department of Pathology, Children's Hospital of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipanjan","family":"Basu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Children's Hospital of Pittsburgh, Pittsburgh, PA, 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