{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:07:13Z","timestamp":1789927633024,"version":"4.0.1"},"reference-count":36,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":3333,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01-CA74177-06 to D.W.C."],"award-info":[{"award-number":["R01-CA74177-06 to D.W.C."]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["ajp.amjpathol.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The American Journal of Pathology"],"published-print":{"date-parts":[[2010,12]]},"DOI":"10.2353\/ajpath.2010.100369","type":"journal-article","created":{"date-parts":[[2010,10,29]],"date-time":"2010-10-29T22:57:47Z","timestamp":1288393067000},"page":"3125-3132","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"title":["Nf1\u2212\/\u2212 Schwann Cell-Conditioned Medium Modulates Mast Cell Degranulation by c-Kit-Mediated Hyperactivation of Phosphatidylinositol 3-Kinase"],"prefix":"10.1016","volume":"177","author":[{"given":"Shi","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Burgin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"McDaniel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleed","family":"Khalaf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Wade","family":"Clapp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Chun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.2353\/ajpath.2010.100369_bib1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0092-8674(90)90252-A","article-title":"Deletions and a translocation interrupt a cloned gene at the neurofibromatosis type 1 locus","volume":"62","author":"Viskochil","year":"1990","journal-title":"Cell"},{"key":"10.2353\/ajpath.2010.100369_bib2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1126\/science.2134734","article-title":"Type 1 neurofibromatosis gene: identification of a large transcript disrupted in three NF1 patients","volume":"249","author":"Wallace","year":"1990","journal-title":"Science"},{"key":"10.2353\/ajpath.2010.100369_bib3","first-page":"149","article-title":"Models for gain-of-function and loss-of-function of MMPs. Transgenic and gene targeted mice","volume":"151","author":"Coussens","year":"2001","journal-title":"Methods Mol Biol"},{"key":"10.2353\/ajpath.2010.100369_bib4","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1126\/science.1067100","article-title":"Matrix metalloproteinase inhibitors and cancer: trials and tribulations","volume":"295","author":"Coussens","year":"2002","journal-title":"Science"},{"key":"10.2353\/ajpath.2010.100369_bib5","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0092-8674(00)81683-9","article-title":"The hallmarks of cancer","volume":"100","author":"Hanahan","year":"2000","journal-title":"Cell"},{"key":"10.2353\/ajpath.2010.100369_bib6","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1126\/science.1068452","article-title":"Neurofibromas in NF1: Schwann cell origin and role of tumor environment","volume":"296","author":"Zhu","year":"2002","journal-title":"Science"},{"key":"10.2353\/ajpath.2010.100369_bib7","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.cell.2008.08.041","article-title":"Nf1-dependent tumors require a microenvironment containing Nf1+\/\u2212 and c-kit-dependent bone marrow","volume":"135","author":"Yang","year":"2008","journal-title":"Cell"},{"key":"10.2353\/ajpath.2010.100369_bib8","first-page":"996","article-title":"Possible involvement of c-kit receptor and its ligand in increase of mast cells in neurofibroma tissues","volume":"117","author":"Hirota","year":"1993","journal-title":"Arch Pathol Lab Med"},{"key":"10.2353\/ajpath.2010.100369_bib9","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1172\/JCI19195","article-title":"Neurofibromin-deficient Schwann cells secrete a potent migratory stimulus for Nf1+\/\u2212 mast cells","volume":"112","author":"Yang","year":"2003","journal-title":"J Clin Invest"},{"key":"10.2353\/ajpath.2010.100369_bib10","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/ng0794-353","article-title":"Tumour predisposition in mice heterozygous for a targeted mutation in Nf1","volume":"7","author":"Jacks","year":"1994","journal-title":"Nat Genet"},{"key":"10.2353\/ajpath.2010.100369_bib11","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1084\/jem.187.11.1893","article-title":"Nf1 regulates hematopoietic progenitor cell growth and ras signaling in response to multiple cytokines","volume":"187","author":"Zhang","year":"1998","journal-title":"J Exp Med"},{"key":"10.2353\/ajpath.2010.100369_bib12","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1182\/blood-2005-05-1985","article-title":"Genetic reduction of class IA PI-3 kinase activity alters fetal hematopoiesis and competitive repopulating ability of hematopoietic stem cells in vivo","volume":"107","author":"Haneline","year":"2006","journal-title":"Blood"},{"key":"10.2353\/ajpath.2010.100369_bib13","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1182\/blood-2004-10-4041","article-title":"p85\u03b1 subunit of class IA PI-3 kinase is crucial for macrophage growth and migration","volume":"106","author":"Munugalavadla","year":"2005","journal-title":"Blood"},{"key":"10.2353\/ajpath.2010.100369_bib14","doi-asserted-by":"crossref","first-page":"3538","DOI":"10.1182\/blood-2004-05-2021","article-title":"K-Ras is essential for normal fetal liver erythropoiesis","volume":"105","author":"Khalaf","year":"2005","journal-title":"Blood"},{"key":"10.2353\/ajpath.2010.100369_bib15","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1042\/bst0270624","article-title":"Phosphoinositide 3-kinase knockout mice: role of p85\u03b1 in B cell development and proliferation","volume":"27","author":"Fruman","year":"1999","journal-title":"Biochem Soc Trans"},{"key":"10.2353\/ajpath.2010.100369_bib16","doi-asserted-by":"crossref","first-page":"48453","DOI":"10.1074\/jbc.M305602200","article-title":"Positive and negative roles of p85\u03b1 and p85\u03b2 regulatory subunits of phosphoinositide 3-kinase in insulin signaling","volume":"278","author":"Ueki","year":"2003","journal-title":"J Biol Chem"},{"key":"10.2353\/ajpath.2010.100369_bib17","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S1074-7613(00)80207-1","article-title":"Rac2 stimulates Akt activation affecting BAD\/Bcl-XL expression while mediating survival and actin function in primary mast cells","volume":"12","author":"Yang","year":"2000","journal-title":"Immunity"},{"key":"10.2353\/ajpath.2010.100369_bib18","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1177\/002215549904700504","article-title":"Enzyme histochemistry of tryptase in stomach mucosal mast cells of the mouse","volume":"47","author":"Valchanov","year":"1999","journal-title":"J Histochem Cytochem"},{"key":"10.2353\/ajpath.2010.100369_bib19","doi-asserted-by":"crossref","first-page":"6768","DOI":"10.4049\/jimmunol.172.11.6768","article-title":"Differential requirement for adapter proteins Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa and adhesion- and degranulation-promoting adapter protein in Fc\u03b5RI signaling and mast cell function","volume":"172","author":"Wu","year":"2004","journal-title":"J Immunol"},{"key":"10.2353\/ajpath.2010.100369_bib20","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1084\/jem.191.1.181","article-title":"Genetic and biochemical evidence that haploinsufficiency of the Nf1 tumor suppressor gene modulates melanocyte and mast cell fates in vivo","volume":"191","author":"Ingram","year":"2000","journal-title":"J Exp Med"},{"key":"10.2353\/ajpath.2010.100369_bib21","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1182\/blood-2008-06-160861","article-title":"p21-activated kinase regulates mast cell degranulation via effects on calcium mobilization and cytoskeletal dynamics","volume":"113","author":"Allen","year":"2009","journal-title":"Blood"},{"key":"10.2353\/ajpath.2010.100369_bib22","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1091\/mbc.6.9.1145","article-title":"Wortmannin blocks lipid and protein kinase activities associated with PI 3-kinase and inhibits a subset of responses induced by Fc\u03b5R1 cross-linking","volume":"6","author":"Barker","year":"1995","journal-title":"Mol Biol Cell"},{"key":"10.2353\/ajpath.2010.100369_bib23","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1091\/mbc.8.5.909","article-title":"c-kit receptor signaling through its phosphatidylinositide-3\u2032-kinase-binding site and protein kinase C: role in mast cell enhancement of degranulation, adhesion, and membrane ruffling","volume":"8","author":"Vosseller","year":"1997","journal-title":"Mol Biol Cell"},{"key":"10.2353\/ajpath.2010.100369_bib24","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1159\/000237486","article-title":"Effects of phosphatidylinositol-3-kinase inhibitors on degranulation and gene induction in allergically triggered mouse mast cells","volume":"112","author":"Pendl","year":"1997","journal-title":"Int Arch Allergy Immunol"},{"key":"10.2353\/ajpath.2010.100369_bib25","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.4049\/jimmunol.178.4.2527","article-title":"K-ras is critical for modulating multiple c-kit-mediated cellular functions in wild-type and Nf1+\/\u2212 mast cells","volume":"178","author":"Khalaf","year":"2007","journal-title":"J Immunol"},{"key":"10.2353\/ajpath.2010.100369_bib26","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1126\/science.283.5400.390","article-title":"Xid-like immunodeficiency in mice with disruption of the p85\u03b1 subunit of phosphoinositide 3-kinase","volume":"283","author":"Suzuki","year":"1999","journal-title":"Science"},{"key":"10.2353\/ajpath.2010.100369_bib27","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0092-8674(00)00139-2","article-title":"MMP-9 supplied by bone marrow-derived cells contributes to skin carcinogenesis","volume":"103","author":"Coussens","year":"2000","journal-title":"Cell"},{"key":"10.2353\/ajpath.2010.100369_bib28","doi-asserted-by":"crossref","first-page":"F23","DOI":"10.1084\/jem.193.6.F23","article-title":"Inflammatory cells and cancer: think different!","volume":"193","author":"Coussens","year":"2001","journal-title":"J Exp Med"},{"key":"10.2353\/ajpath.2010.100369_bib29","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/nature01322","article-title":"Inflammation and cancer","volume":"420","author":"Coussens","year":"2002","journal-title":"Nature"},{"key":"10.2353\/ajpath.2010.100369_bib30","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.1182\/blood.V75.11.2204.2204","article-title":"Degranulating mast cells secrete an endoglycosidase that degrades heparan sulfate in subendothelial extracellular matrix","volume":"75","author":"Bashkin","year":"1990","journal-title":"Blood"},{"key":"10.2353\/ajpath.2010.100369_bib31","first-page":"315","article-title":"Effect of the mast cell activator compound 48\/80 and heparin on angiogenesis in the chick chorioallantoic membrane","volume":"7","author":"Clinton","year":"1988","journal-title":"Int Microcirc Clin Exp"},{"key":"10.2353\/ajpath.2010.100369_bib32","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1016\/j.ajog.2005.04.055","article-title":"Neovascularization and mast cells with tryptase activity increase simultaneously with pathologic progression in human endometrial cancer","volume":"193","author":"Ribatti","year":"2005","journal-title":"Am J Obstet Gynecol"},{"key":"10.2353\/ajpath.2010.100369_bib33","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1002\/jnr.20648","article-title":"c-Kit receptor expression in normal human Schwann cells and Schwann cell lines derived from neurofibromatosis type 1 tumors","volume":"82","author":"Dang","year":"2005","journal-title":"J Neurosci Res"},{"key":"10.2353\/ajpath.2010.100369_bib34","doi-asserted-by":"crossref","first-page":"5677","DOI":"10.1158\/1078-0432.CCR-03-0769","article-title":"Circulating growth factor levels are associated with tumorigenesis in neurofibromatosis type 1","volume":"10","author":"Mashour","year":"2004","journal-title":"Clin Cancer Res"},{"key":"10.2353\/ajpath.2010.100369_bib35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1084\/jem.194.1.57","article-title":"Hyperactivation of p21ras and the hematopoietic-specific Rho GTPase. Rac2, cooperate to alter the proliferation of neurofibromin-deficient mast cells in vivo and in vitro","volume":"194","author":"Ingram","year":"2001","journal-title":"J Exp Med"},{"key":"10.2353\/ajpath.2010.100369_bib36","doi-asserted-by":"crossref","first-page":"4646","DOI":"10.1182\/blood-2008-04-155085","article-title":"(2008). Pak1 regulates multiple c-Kit mediated Ras-MAPK gain-in-function phenotypes in Nf1+\/\u2212 mast cells","volume":"112","author":"McDaniel","year":"2008","journal-title":"Blood"}],"container-title":["The American Journal of Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002944010629367?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0002944010629367?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T14:19:18Z","timestamp":1757600358000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0002944010629367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["S0002944010629367"],"URL":"https:\/\/doi.org\/10.2353\/ajpath.2010.100369","relation":{},"ISSN":["0002-9440"],"issn-type":[{"value":"0002-9440","type":"print"}],"subject":[],"published":{"date-parts":[[2010,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nf1\u2212\/\u2212 Schwann Cell-Conditioned Medium Modulates Mast Cell Degranulation by c-Kit-Mediated Hyperactivation of Phosphatidylinositol 3-Kinase","name":"articletitle","label":"Article Title"},{"value":"The American Journal of Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.2353\/ajpath.2010.100369","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}