{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T08:13:55Z","timestamp":1761898435164,"version":"build-2065373602"},"reference-count":40,"publisher":"The Korean Society of Nephrology","issue":"6","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Korean Fund for Regenerative Medicine"},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["23A0201L1"],"award-info":[{"award-number":["23A0201L1"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1A2C3007759"],"award-info":[{"award-number":["2020R1A2C3007759"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["krcp-ksn.org"],"crossmark-restriction":true},"short-container-title":["Kidney Res Clin Pract"],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:p>Background: Stem cell-based therapy is one of the tools for acute kidney injury (AKI) treatment. Tonsil tissue is a promising alternative source for the high-yield isolation of mesenchymal stem cells (MSCs). This study was undertaken to investigate the effects of tonsil-derived MSCs (T-MSCs) in animal model of AKI induced by gentamicin (GM).Methods: Twenty Sprague-Dawley rats were divided into four groups: Control, GM (70 mg\/kg\/day, intraperitoneal injection for 10 days), GM + T-MSCs (1 \u00d7 107 cells, intravenous injection at 1 day after the last vehicle\/GM), and T-MSCs. Renal function, apoptosis, and markers of endoplasmic reticulum stress were measured on day 16 after the first vehicle\/GM. Oxidative stress was assessed by measuring urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and the expression of glutathione peroxidase (GPx) and catalase. Effects of T-MSCs on GM-induced apoptosis and oxidative stress in NRK cells were also evaluated using a co-culture technique of NRK cells and T-MSC.Results: In the GM + T-MSCs group, blood urea nitrogen, creatinine, and tubular damage score were lower compared to the GM group. T-MSCs injection decreased apoptotic cells and the expression of Bax, cytochrome c, and cleaved caspase and increased Bcl-2. T-MSC injection decreased urinary 8-OHdG and increased expression of GPx and catalase in the kidneys. Anti-human nuclei and PKH26 staining demonstrated the localization of T-MSCs in the tubules of renal cortex. In-vitro study revealed that T-MSCs or T-MSC-conditioned media ameliorated GM-induced nicotinamide adenine dinucleotide phosphate oxidase-1 expression, hydrogen peroxide generation, and apoptosis of NRK cells. Conclusion: Our study demonstrated that T-MSCs ameliorated GM-induced AKI by directly incorporating into the damaged renal tubules and exerting antiapoptotic and antioxidative effects.<\/jats:p>","DOI":"10.23876\/j.krcp.24.234","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T06:53:09Z","timestamp":1754981589000},"page":"899-915","update-policy":"https:\/\/doi.org\/10.23876\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tonsil-derived mesenchymal stem cells protect the kidney from gentamicin-induced acute kidney injury by incorporation into damaged renal tubules and amelioration of oxidative and endoplasmic reticulum stresses"],"prefix":"10.23876","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4977-2741","authenticated-orcid":true,"given":"Mina","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9454-783X","authenticated-orcid":true,"given":"Dal-Ah","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3055-1757","authenticated-orcid":true,"given":"Eun-Sun","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9691-8948","authenticated-orcid":true,"given":"Sung Min","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3174-8965","authenticated-orcid":true,"given":"Sung-Chul","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6060-6416","authenticated-orcid":true,"given":"Inho","family":"Jo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2239-0225","authenticated-orcid":true,"given":"Han Su","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8475-8932","authenticated-orcid":true,"given":"Duk-Hee","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"9960","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1681\/asn.2005091017"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109649"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-021-06454-7"},{"key":"ref4","first-page":"1","volume-title":"Kidney disease: Improving global outcomes (KDIGO) acute kidney injury work group. KDIGO clinical practice guideline for acute kidney injury","author":"Kellum","year":"2012"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2013.1517"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2012.08.034"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3109"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/srep20287"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1097\/01.asn.0000128974.07460.34"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1172\/jci23015"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2009.12.003"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.105"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002\/stem.3058"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1186\/ar2459"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2012.706708"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.06.007"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24690"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183141"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2014.101"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2010.337"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2012.04.016"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfm152"},{"key":"ref23","first-page":"1","volume-title":"Comparison of stem cell therapies for acute kidney injury","author":"Barnes","year":"2016"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0795"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1379680"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2020-047622"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.03.006"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.7.1115"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2015.08.001"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2016.0063"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0200111"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1681\/asn.2008070798"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2015.2505"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1002\/stem.293"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc.2013.6.1.55"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.16-0363"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2017.04.015"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17111826"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2021.02.001"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.3390\/cells8040368"}],"container-title":["Kidney Research and Clinical Practice"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/krcp-ksn.org\/upload\/pdf\/j-krcp-24-234.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/krcp-ksn.org\/journal\/view.php?doi=10.23876\/j.krcp.24.234","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/krcp-ksn.org\/upload\/pdf\/j-krcp-24-234.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:25:29Z","timestamp":1761895529000},"score":1,"resource":{"primary":{"URL":"http:\/\/krcp-ksn.org\/journal\/view.php?doi=10.23876\/j.krcp.24.234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,1]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"URL":"https:\/\/doi.org\/10.23876\/j.krcp.24.234","relation":{},"ISSN":["2211-9140"],"issn-type":[{"type":"electronic","value":"2211-9140"}],"subject":[],"published":{"date-parts":[[2025,11,1]]},"assertion":[{"value":"2024-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-11","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}