{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T07:47:59Z","timestamp":1782719279412,"version":"3.54.5"},"reference-count":36,"publisher":"Korea Disease Control and Prevention Agency","issue":"3","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Ministry of Higher Education, Science, and Technology, Indonesia","award":["115\/C3\/DT.05.00\/PL\/2025"],"award-info":[{"award-number":["115\/C3\/DT.05.00\/PL\/2025"]}]}],"content-domain":{"domain":["ophrp.org"],"crossmark-restriction":true},"short-container-title":["PHRP"],"published-print":{"date-parts":[[2026,6,30]]},"abstract":"<jats:p>Objectives: &lt;i&gt;Klebsiella pneumoniae&lt;\/i&gt; is a leading cause of hospital-acquired infections. Its increasing prevalence poses substantial challenges to both hospital and public health systems due to the emergence of multidrug-resistant strains. Understanding the epidemiology of &lt;i&gt;K. pneumoniae&lt;\/i&gt; and its antimicrobial resistance characteristics may support antimicrobial stewardship and infection control programs. A cross-sectional study was conducted from June to November 2025.Methods: A total of 62 isolates underwent phenotypic identification and antimicrobial susceptibility testing using the BD Phoenix system (Becton Dickinson), followed by molecular detection of &lt;i&gt;K. pneumoniae&lt;\/i&gt; and efflux pump genes. Sanger sequencing was performed on isolates positive for the &lt;i&gt;tolC&lt;\/i&gt; gene.Results: Among the isolates, 66.1% were classified as extended-spectrum \u03b2-lactamase (ESBL) producers, 6.5% as carbapenem-resistant Enterobacterales (CRE), and 6.5% as both ESBL and CRE producers. Most clinical isolates were resistant to ampicillin, cefazolin, ceftazidime, ceftriaxone, ciprofloxacin, and cefepime. The &lt;i&gt;acrAB&lt;\/i&gt; and &lt;i&gt;tolC&lt;\/i&gt; genes were detected in all 62 isolates. One isolate showed a genetic profile similar to that of the &lt;i&gt;K. pneumoniae&lt;\/i&gt; KP 52.145 strain.Conclusion: This study demonstrated a high prevalence of antibiotic resistance among &lt;i&gt;K. pneumoniae&lt;\/i&gt; isolates and confirmed the presence of efflux pump genes, including &lt;i&gt;tolC&lt;\/i&gt;, with observable genetic variability. Further investigation of &lt;i&gt;tolC&lt;\/i&gt; gene evolution is essential, as these genes play critical roles in antibiotic resistance mechanisms.<\/jats:p>","DOI":"10.24171\/j.phrp.2025.0573","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T06:12:30Z","timestamp":1776665550000},"page":"254-261","update-policy":"https:\/\/doi.org\/10.24171\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic profiling of the tolC component of the acrAB-TolC efflux pump in clinical Klebsiella pneumoniae isolates: a cross-sectional study in Pontianak, Indonesia"],"prefix":"10.24171","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8847-6780","authenticated-orcid":true,"given":"Mardhia","family":"Mardhia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9799-5459","authenticated-orcid":true,"given":"Delima 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