{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T04:09:40Z","timestamp":1742530180531,"version":"3.40.1"},"reference-count":1,"publisher":"Polish Academy of Sciences Chancellery","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"Honey is natural nectar that honey bees gather from a variety of flowers. Honey is made up of many nutrients that are essential for the growth and development of various tissues and organs in all kinds of living organisms. The goal of the current study was to determine whether adding honey to diets of naked neck cocks would have a good impact on their semen quality, haematological profile, and immune system. A total of 90 naked neck cocks that were 75 weeks old were gathered for this purpose in March and April 2021 from the University of Agriculture Birds Stock. The birds were divided into 3 equal treatment groups: control, A and B, which each received a dose of 5 and 10 g of honey in 1 litre of water (w\/v), respectively. The control group received no treatment. Five weeks of treatment were given, with one week serving as an adaptation period. One-way analysis of variance was applied to the recorded data for statistical analysis. The addition of honey to drinking water at a dose of 10 g (w\/v) increased (p&lt;0.05) sperm motility, followed by a dose of 5 g (w\/v) and the control group, but the mean values of non-motile sperms were lowest (p&lt;0.05) at a dose of 10 g (w\/v), followed by a dose of 5 g (w\/v), and the control group. However, there were no significant differences in semen volume, pH, or concentration across the treatment groups. The haematological parameters, such as haemoglobin, total leukocyte count, red blood cells, and packed cell volume, were not significantly affected by the addition of honey to the drinking water, but the ND titer of naked neck cocks treated with 10 g (w\/v) honey was significantly (p&lt;0.05) higher than that of the cocks in the 5 g (w\/v) and control group. In conclusion, the addition of honey to drinking water at a dose of 10 g (w\/v) may have positive effects on sperm motility and antibody titer against Newcastle disease in aged naked neck cocks. Furthermore, these results also suggest that the addition of honey to drinking water at a dose of 10 g (w\/v) may rejuvenate naked neck cocks even at old age (75 week age).<\/jats:p>","DOI":"10.24425\/pjvs.2025.154012","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T10:27:23Z","timestamp":1742466443000},"page":"43","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating the impact of honey inclusion in drinking water on the semen quality, immunological response, and haematology of naked neck cocks"],"prefix":"10.24425","author":[{"given":"Z.","family":"Farooq","sequence":"first","affiliation":[{"name":"Department of Zoology, Faculty of Biosciences, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.M.","family":"Salam","sequence":"additional","affiliation":[{"name":"Livestock and Dairy Development Department, Poultry Research Institute, Rawalpindi-46300, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Qumar","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.S.","family":"Magsi","sequence":"additional","affiliation":[{"name":"Department of Dairy Technology, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand-67210, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.T.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Raziq","sequence":"additional","affiliation":[{"name":"Department of Livestock and Dairy Development (Extension), KPK \u2013 Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Liaqat","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Faran","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Biochemistry, Biotechnology and Bioinformatics, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Bughio","sequence":"additional","affiliation":[{"name":"Department of Poultry Production, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand-67210, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, Faculty of Animal Husbandry and Veterinary Science, The University of Peshawar-25130, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Rauf","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Science, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"Gondal","sequence":"additional","affiliation":[{"name":"Institute of Continuing Education and Extension, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Arslan","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Abbas","sequence":"additional","affiliation":[{"name":"Department of Animal Production, Riphah College of Veterinary Sciences, Lahore-54000, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.M.","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Livestock Management, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Azhar","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Wadood","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Asad","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur-63100, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"37468","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"ref0","unstructured":"Abioja MO, Akinjute OF, Balogun SI, Oguntimehin MS, Oluwasola TO (2019) Effect of Honey on Haematology, Plasma Biochemistry and Liver Enzymes in Broiler Chickens Administered Dietary Corticosterone. J Stress Physiol Biochem 15: 5-13. Abuoghaba AA, Ismail, II (2018) Impact of bee pollen supplementation on productive performance, some hematological parameters, blood constituents and semen physical characteristics of Sinai chickens. Egypt Poult Sci J 38: 621-635. Adebiyi FM, Akpan I, Obiajunwa EI, Olaniyi HB (2004) Chemical\/physical characterization of Nigerian honey. Pak J Nutr 3: 278-281. Adekunle MO, Abioja MO, Abiona JA, Jegede AV, Sodipe OG (2017) Rectal temperature, heart rate, packed cell volume and differential white blood cell count of laying pullets to honey supplemented water during hot \u2013 dry season. Slovak J Anim Sci 50: 15-20. Ahmed M, Djebli N, Aissat S, Khiati B, Meslem A, Bacha S (2013) In vitro activity of natural honey alone and in combination with curcuma starch against Rhodotorula mucilaginosa in correlation with bioactive compounds and diastase activity. Asian Pac J Trop Biomed 3: 816-821. Ajibola CF, Fashakin JB, Fagbemi TN, Aluko RE (2013) Renin and angiotensin converting enzyme inhibition with antioxidant properties of African yam bean protein hydrolysate and reverse-phase HPLC-separated peptide fractions. Food Res Int 2: 437-444. Alexander DJ, Chettle NJ (1977) Procedures for the haemagglutination and the haemagglutination inhibition tests for avian infectious bronchitis virus. Avian Pathology 6: 9-17. Alvarez-Suarez JM, Gasparrini M, Forbes-Hern\u00e1ndez TY, Mazzoni L, Giampieri F (2014) The composition and biological activity of honey: a focus on Manuka honey. Foods 3: 420-432. Attia YA, Abd Al-Hamid AE, Ibrahim MS, Al-Harthi MA, Bovera F, Elnaggar AS (2014) Productive performance, biochemical and hematological traits of broiler chickens supplemented with propolis, bee pollen, and mannan oligosaccharides continuously or intermittently. Livest Sci 164: 87-95. Awad F, Forrester A, Baylis M, Lemiere S, Jones R, Ganapathy K (2015) Immune responses and interactions following simultaneous application of live Newcastle disease, infectious bronchitis and avian metapneumovirus vaccines in specific-pathogen-free chicks. Res Vet Sci 98: 127-133. Bradely TJ (2008) Active transport in insect recta. J Exp Biol 211: 835-836. Elnagar SA (2010) Royal jelly counteracts bucks\u2019\u201csummer infertility\u201d. Anim Reprod Sci 121: 174-180. Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E (2008) Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem Toxicol 46: 3774-3779. Farag SA, El-Rayes T (2016) Research article effect of bee-pollen supplementation on performance, carcass traits and blood parameters of broiler chickens. Asian J Anim Vet Adv 11: 168-177. Flecknell PA (1979) Clinical, biochemical and haematological reference values in normal experimental animals. J Clin Pathol 32: 96. Freitas JA, Vanat N, Pinheiro JW, Balarin MR, Sforcin JM, Venancio EJ (2011) The effects of propolis on antibody production by laying hens. Poult Sci 90: 1227-1233. Hafez ESE (1978) Reproduction in farm animals. 2nd ed., PP: 237. Hegazi A, Abdou M, Allah FA (2013) Influence of honey on immune response against Newcastle disease vaccine. Int J Basic Appl Virol 2: 1-5. Igbokwe VU, Gege-Adebayo GI, Ogbadu S (2013) Pure honey a potent fertility booster: activities of honey on sperm parameters in young adult rats. IOSR J Dent Med Sci 9: 43-47. Khadr AH, Abdou A, El-Sherbiny AM (2015) Age of puberty and fertility of male New Zealand white rabbits orally administered with royal jelly or\/ and bee honey. J Anim Poult Prod 6: 201-217. Khan MT, Mehmood S, Mahmud A, Javed K (2019) Performance traits, blood biochemistry, immune response and economic appraisal of broilers fed different levels of poultry byproducts compost. J Anim Plant Sci 29: 1549-1557. Mahmood HMA, Al-Daraji HJ (2011) Effect of dietary zinc supplementation onsome seminal plasma characteristics of broiler breeders\u2019 male. Int J Poult Sci 10: 814-818. Mandal MD, Mandal S (2011) Honey: its medicinal property and antibacterial activity. Asian Pac J Trop Biomed 1: 154-160. Mashaly MM, Hendricks 3rd GL, Kalama MA, Gehad AE, Abbas AO, Patterson PH (2004) Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult Sci 83: 889-894. Memon SS, Kamboh AA, Leghari IH, Leghari RA (2019) Effect of in ovo and post-hatch administration of honey on the immunity and intestinal microflora of growing chickens. J Anim Feed Sci 28: 346-353. Memon SS, Kamboh AA, Khand FM, Malhi KK, Leghari A, Lakho SA, Arain MB (2021) Effect of in ovo honey administration on fertility and post hatch performance of broiler chickens. J Anim Health Prod 9: 236-242. Michalkiewicz A, Biesaga M, Pyrzynska K (2008) Solid-phase extraction procedure for determination of phenolic acids and some flavonols in honey. J Chromatogr A 1187: 18-24. Obun CO, Yahaya MS, Olafadehan OA, Kehinde AS, Allison DS, Yusuf AM, Farouk IU (2008) Dietary value of honey and it effects on abdominal fat deposit, blood and serum profile of finisher broiler chicks. J Agric For Soc Sci 6:1-7. Oke OE, Sorungbe FO, Abioja MO, Oyetunji O, Onabajo AO (2016) Effect of different levels of honey on physiological, growth and carcass traits of broiler chickens during dry season. Acta Agriculturae Slovenica 108: 45-53. Osakwe I, Igwe R (2015) Physiological responses of laying birds fed honey and vitamin C in drinking water. In Conference on International Research on Food Security, Natural Resource Management and Rural Development. Humboldt-Universit\u00e4t zu Berlin and the Leibniz Centre for Agricultural Landscape Research (ZALF) Tropentag, Berlin, Germany, September, pp 16-18. Osunkeye OJ, Fakolade PO, Alabi BO, Akinduro VO, Olorede BE (2016) Physiological, Serum and Haematological Responses of Broiler Fed Honey at Varying Levels of Inclusion in the Diet. J Na Sci Res 6: 2225-0921. Rabie AH, Stino FR, El-Kaiaty AM (2018) Influence of some honey bee products on some haematological and immunological parameters and meat quality in broilers. Egypt Poult Sci J 38: 533-549. Ramadan MF, Al-Ghamdi A (2012) Bioactive compounds and health-promoting properties of royal jelly: A review. J Funct Foods 4: 39-52. Saeed M, Arain MA, Kamboh AA, Memon SA, Umar M, Rashid M, Babazadeh D, El-Hack ME, Alagawany M (2017) Raw propolis as a promising feed additive in poultry nutrition: trends and advances. J Anim Health Prod 5: 132-142. SAS Institute (2002) User Guide: Statistics. Version 9.01. Cary, NC, USA: SAS Institute. Shreif EY, El-Saadany AS (2017) Effect of supplementing diet with propolis on Bandarah chicks\u2019 performance. Egypt Poult Sci J 37: 169-184. Steel RGD, Torrie JH (1980) Principles and procedures of Statistics. McGraw-Hill, New York, USA. Syazana N, Hashida N, Majid A, Durriyyah H, HA DS, Kamaruddin M (2011) Effects of gelam honey on sperm quality and testis of Rat. Sains Malaysiana 40: 1243-1246. Tan HT, Rahman RA, Gan SH, Halim AS, Asma\u2019Hassan S, Sulaiman SA, Kirnpal-Kaur BS (2009) The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complement Altern Med 9: 34. USDA (2014) Nutrient data for honey. 2014. USDA nutrient data laboratory, version SR- 27, in USDA National Nutrient Database for Standard Reference. Viuda\u2010Martos M, Ruiz\u2010Navajas Y, Zaldivar\u2010Cruz JM, Kuri V, Fern\u00e1ndez\u2010L\u00f3pez J, Carbonell\u2010Barrachina \u00c1A, P\u00e9rez\u2010 -\u00c1lvarez J (2010) Aroma profile and physico\u2010chemical properties of artisanal honey from Tabasco, Mexico. Int J Food Sci Technol 45: 1111-1118. Wang YC, Li GH, Lu JB, Zhu RL (2005) Impact of broken wall bee pollen on immune function and intestinal bacterium group of chickens. Acta Ecologiae Animalis Domastici 26: 17-21. Washburn KW, Stino FKR (1973) Changes in reproductive traits associated with selection for packed erythrocyte volume in Japanese quail. Poult Sci 52: 2147-2152. Zafarnejad K, Afzali N, Rajabzadeh M (2017) Effect of bee glue on growth performance and immune response of broiler chickens. J Appl Anim Res 45: 280-284. Zemjanis R (1970) Collection and evaluation of semen. In: diagnostic and therapeutic technique in animal reproduction. 2nd ed., Williams and Wilson Co., Baltimore, MD, 139-156."}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"language":"pl","deposited":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T10:50:22Z","timestamp":1742467822000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.pan.pl\/dlibra\/journal\/154007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,20]]},"references-count":1,"URL":"https:\/\/doi.org\/10.24425\/pjvs.2025.154012","relation":{},"ISSN":["1505-1773"],"issn-type":[{"value":"1505-1773","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3,20]]}}}