{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T15:10:07Z","timestamp":1742483407929,"version":"3.40.1"},"reference-count":1,"publisher":"Polish Academy of Sciences Chancellery","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Traumatic ulcers are oral mucosa lesions caused by thermal, mechanical, and chemical factors. Long-term topical medication use can lead to fungal resistance. Patin fish skin contains glycine and glutamic acid, which can enhance macrophage activation and fibroblast proliferation, aiding wound healing. This study aimed to determine the effect of Patin skin gelatin on increasing macrophages and fibroblasts and reducing the diameter of traumatic ulcers in Wistar rats. An experimental laboratory study with a post-test-only control group design was conducted. Ulcers were induced on the labial mucosa of the mandible in rats using thermal induction. Treatments included a placebo gel and Patin skin gelatin at concentrations of 10%, 20%, and 30% for 7 days. Rats were euthanized on days 3, 5, and 7, and ulcerated mucosal tissue was biopsied to count macrophages and fibroblasts using an Olympus microscope. Results showed significant macrophage and fibroblast count differences between the gelatin and placebo treatment groups. Patin skin gelatin at all concentrations increased the number of fibroblasts and decreased ulcer diameters significantly compared to the control group (p&amp;lt;0.05). Patin skin gelatin effectively enhanced fibroblast cell proliferation and reduced ulcer size over the 7-day treatment period. The 10% concentration of Patin skin gelatin showed the most significant increase in fibroblast count and the greatest reduction in ulcer diameter.<\/jats:p>","DOI":"10.24425\/pjvs.2025.154023","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T13:02:32Z","timestamp":1742475752000},"page":"151-151","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of Patin (Pangasius sp.) gelatin on macrophage and fibroblast proliferation in traumatic ulcer rats"],"prefix":"10.24425","author":[{"given":"A.","family":"Puspa Sari","sequence":"first","affiliation":[{"name":"Faculty of Medicine, University of Sriwijaya, Jl. Dr Muhammad Ali, Sekip Jaya, Palembang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Prima Anugrah","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Sriwijaya, Jl. Dr Muhammad Ali, Sekip Jaya, Palembang, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Novita","sequence":"additional","affiliation":[{"name":"Primatology Study Program, Graduate School of IPB University, Jl. Lodaya II No 5, Bogor, Indonesia"},{"name":"Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Institute Research, and Innovation Agency, Jl. Raya Bogor-Jakarta KM. 46, Cibinong, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"37468","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Amtha R, Marcia M, Aninda A (2017) Thrush plasters effectively accelerate the healing of recurrent aphthous stomatitis and traumatic ulcers. Maj Kedokt Gigi Indones 3: 69. https:\/\/doi.org\/10.22146\/majkedgiind.22097 Fatimatuzzahro N, Pujiastuti P, Alicia RS (2021) Potential of 5% Argiope modesta spiders cocoon extract gel on the fibroblasts number and collagen density. J Kedokt Gigi Univ Padjadjaran 33: 233. Gouin JP, Kiecolt-Glaser JK (2011) The Impact of Psychological Stress on Wound Healing: Methods and Mechanisms. Immunol Allergy Clin North Am 31: 81-93. Guo S, DiPietro LA (2010) Critical review in oral biology &amp; medicine: Factors affecting wound healing. J Dent Res 89: 219-229. Ito R, Morimoto N, Pham LH, Taira T, Kawai K, Suzuki S (2013) Efficacy of the controlled release of concentrated platelet lysate from a collagen gelatin scaffold for dermislike tissue regeneration. Tissue Eng Part A 19: 1398-1405. Laskaris G (2003) Color Atlas of Oral Diseases. 3rd edition. Thieme: New York Mardiyantoro F, Andriani D (2019) The effect of Patin (Pangasius Djambal) gelatin on the number of fibroblasts in wounds after tooth extraction in Rats (Rattus norvegicus). [article in Indonesian]. Odonto Dent J 6: 1-5. Pratiwi L, Prasetyawan S, Vidiastuti D (2020) The Effect of Hydrolyzed Fish Collagen Ointment to Healing Burns Level IIB by Fibroblast Growth Factor 2 (FGF-2) and Fibroblast in Rats (Rattis norvegicus). MKH 31: 52. [Available at https:\/\/e-journal.unair.ac.id\/MKH\/index] Rosada A, Mujayanto R, Poetri AR (2020) Salam Leaf Extract in Increasing Fibroblast Growth Factor Expression in Traumatic Ulcers of the Oral Cavity. Odonto in Dent J 7: 90. http:\/\/dx.doi.org\/10.30659\/odj.7.2.90-96 See SF, Hong PK, Ng KL, Wan Aida, and Babji AS (2010) Physicochemical properties of gelatins extracted from skins of different freshwater fish species. Int Food Res J 17: 809-816 Siregar FM (2019) Immunosenescence\u202f: Aging of Macrophage Cells. [article in Indonesian]. J Ilmu Kedokt 13: 14. Sun M, Xie Q, Cai X, Liu Z, Wang Y, Dong X, Xu Y (2020) Preparation and characterization of epigallocatechin gallate, ascorbic acid, gelatin, and chitosan nanoparticles and their beneficial effect on wound healing of diabetic mice. Int J Biol Macromol 148: 777-784. Suptijah P, Indriani D, Wardoyo SE (2018) Isolation and Characterization of Collagen from the Skin of Catfish (Pangasius sp.). J Sains Nat 8: 8. Violeta BV, Hartomo BT (2020) Management of Traumatic Ulcer Care in Traumatic Occlusion Patients: Case Report. [article in Indonesian]. e-GiGi. 8: 86-92. Wardani RP, Kholifa M, Yuletnawati SE (2017) Effect of Lime Peel Ethanol Extract (Citrus aurantifolia (Christm.) Swingle) on Traumatic Ulcer Healing in Rattus norvegicus Wistar Strain. JIKG 1: 23-27. [https:\/\/journals.ums.ac.id\/index.php\/jikg\/article\/view\/4149] Wu S, Deng L, Hsia H, Xu K, He Y, Huang Q, Peng Y, Zhou Z, Peng C (2017) Evaluation of gelatin-hyaluronic acid composite hydrogels for accelerating wound healing. J Biomater Appl 31: 1380-1390. Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y (2011a) Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats. Mar Drugs 9: 696-711. Zhang Z, Wang J, Ding Y, Dai X, Li Y (2011b) Oral administration of marine collagen peptides from Chum Salmon skin enhances cutaneous wound healing and angiogenesis in rats. J Sci Food Agric 91: 2173-2179. Zomer HD, Trentin GA (2018) Skin wound healing in humans and mice: Challenges in translational research. J Dermatol Sci 90: 3-12.","DOI":"10.22146\/majkedgiind.22097"}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"language":"pl","link":[{"URL":"https:\/\/journals.pan.pl\/Content\/134472\/PDF\/16%20_%20Puspa%20Sari.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T14:06:44Z","timestamp":1742479604000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.pan.pl\/dlibra\/publication\/154023\/edition\/134472\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,20]]},"references-count":1,"URL":"https:\/\/doi.org\/10.24425\/pjvs.2025.154023","relation":{},"ISSN":["1505-1773"],"issn-type":[{"type":"print","value":"1505-1773"}],"subject":[],"published":{"date-parts":[[2025,3,20]]}}}