{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T10:24:26Z","timestamp":1758191066602,"version":"3.44.0"},"reference-count":1,"publisher":"Polish Academy of Sciences Chancellery","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"Toll-like receptors (TLRs) are essential components of both innate and adaptive immunity, influencing various physiological and pathological processes. It is also known that immune system components are present in the corpus luteum (CL) and regulate its functions. This study aimed to, for the first time, examine the mRNA expression levels of TLR2, TLR4, TLR7, and interleukin-8 (IL-8) in canine luteal tissue samples collected during early diestrus (EDI) (n=5), early pregnancy (EPR) (n=5), and pyometra (PYO) (n=5), a pathological condition in which the CL remains active. The luteal tissue samples were obtained via ovariohysterectomy and analyzed using real-time quantitative polymerase chain reaction (RT-qPCR). It was found that the mRNA expression levels of TLR2, TLR7, and IL-8 were highest in the PYO group (p&lt;0.05). However, no significant difference was observed in TLR4 mRNA expression between groups (p&gt;0.05). This study reveals distinct expression profiles of TLRs and IL-8 in canine luteal tissue under different physiological and pathological states. In conclusion, pyometra may induce changes in the mRNA expression patterns of TLRs and IL-8 in canine luteal tissues. Further research involving protein-level analyses with larger sample sizes is necessary to better understand the roles of these molecules in the regulation of luteal function.<\/jats:p>","DOI":"10.24425\/pjvs.2025.156068","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T08:27:27Z","timestamp":1758097647000},"page":"429-429","source":"Crossref","is-referenced-by-count":0,"title":["Differential mRNA expression of luteal TLR2, TLR4, TLR7, and interleukin-8 across canine reproductive states"],"prefix":"10.24425","author":[{"given":"C.","family":"Peker","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ayd\u0131n Adnan Menderes University, I\u015f\u0131kl\u0131, 09020, Efeler, Ayd\u0131n, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.H.","family":"U\u00e7ar","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ayd\u0131n Adnan Menderes University, I\u015f\u0131kl\u0131, 09020, Efeler, Ayd\u0131n, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"K\u00f6se","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Dicle University, Fetih, 21200, Sur, Diyarbak\u0131r, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Polat","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences, Dicle University, K\u0131t\u0131lb\u0131l, 21280, Sur, Diyarbak\u0131r, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.O.","family":"Atl\u0131","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Harran University, Asya, 63200, Eyy\u00fcbiye, \u015eanl\u0131urfa, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"37468","published-online":{"date-parts":[[2025,9,16]]},"reference":[{"key":"ref0","unstructured":"Aflatoonian R, Fazeli A (2008) Toll-like receptors in female reproductive tract and their menstrual cycle dependent expression. J Reprod Immunol 77: 7-13. 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A critical re-evaluation of a receptor expressing apparent broad specificity. Immunobiology 213: 205-224.    "}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"language":"pl","link":[{"URL":"https:\/\/journals.pan.pl\/Content\/136420\/PDF\/8%20_%20Peker.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T08:35:38Z","timestamp":1758098138000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.pan.pl\/dlibra\/publication\/156068\/edition\/136420\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,16]]},"references-count":1,"URL":"https:\/\/doi.org\/10.24425\/pjvs.2025.156068","relation":{},"ISSN":["1505-1773"],"issn-type":[{"value":"1505-1773","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9,16]]}}}