{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T20:47:30Z","timestamp":1773866850980,"version":"3.50.1"},"reference-count":2,"publisher":"Polish Academy of Sciences Chancellery","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The aim of this study was to determine the prevalence of thermophilic Campylobacter species in urban wild birds living alongside humans in Istanbul, to determine the species distribution of isolated strains, and to characterize their antimicrobial resistance profiles. In this study, 150 fresh fecal samples from wild birds living alongside humans (crows and seagulls living along the coastlines, and pigeons living in tourist areas in the city center) were collected from various regions of Istanbul, T\u00fcrkiye. For Campylobacter isolation, mCCDA agar was inoculated, and suspected isolates identified as Campylobacter spp. by biochemical tests were identified by PCR. The phenotypic and genotypic antimicrobial susceptibility profiles of the isolated strains were determined. When fecal samples were examined using conventional methods, Campylobacter spp. was isolated from 2\/150 (1.33%). As a result of mPCR applied to DNAs obtained directly from the examination samples, 16 Campylobacter spp. were found in 15\/150 (10%). 14 of these (93.3%) were identified as C. coli, and 2 (12.5%) were identified as C. jejuni. Campylobacter was detected in 11 of 127 pigeons (8.66%), while 10 (7.87%) were identified as C. coli, and 2 (1.57%) were identified as C. jejuni; Campylobacter was detected in 4 of 22 seagulls (18.18%), and all were identified as C. coli. When the sample collection regions were compared, the frequency of Campylobacter spp. was highest in Beyaz\u0131t Square (60%), followed by K\u00fc\u00e7\u00fck\u00e7ekmece Beach (40%). As a result of antimicrobial susceptibility tests and PCR performed for the presence of tet(O), aphA-3 and gyrA genes, gyrA gene was found in both isolates, while tet(O) and aphA-3 genes were not detected. This study revealed that urban wild bird populations resident in Istanbul are a significant reservoir for Thermophilic Campylobacter species and pose a potential public health risk.<\/jats:p>","DOI":"10.24425\/pjvs.2026.158500","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T13:03:47Z","timestamp":1773839027000},"page":"49-49","source":"Crossref","is-referenced-by-count":0,"title":["Zoonotic risk of Campylobacter spp. in urban wild birds: Prevalence and antimicrobial resistance profiles in Istanbul, T\u00fcrkiye"],"prefix":"10.24425","author":[{"given":"A.","family":"S\u0131tk\u0131","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpa\u015fa, Alkent 2000 mah., Yi\u011fitt\u00fcrk cd., B\u00fcy\u00fck\u00e7ekmece, 34500, Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Diren S\u0131\u011f\u0131rc\u0131","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpa\u015fa, Alkent 2000 mah., Yi\u011fitt\u00fcrk cd., B\u00fcy\u00fck\u00e7ekmece, 34500, Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"37468","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"#cr-split#-ref0.1","unstructured":"Abdollahpour N, Zendehbad B, Alipour A, Khayatzadeh J (2015) Wild-bird feces as a source of Campylobacter jejuni infection in children's playgrounds in Iran. Food Control 50: 378-381. Adiguzel MC, Sigirci BD, Celik B, Kahraman BB, Metiner K, Ikiz S, Ozgur NY (2018) Phenotypic and genotypic examination of antimicrobial resistance in thermophilic Campylobacter species isolated from poultry in Turkey. J Vet Res 62: 463-468. Ahmed NA, Gulhan T (2022) Campylobacter in Wild Birds: Is It an Animal and Public Health Concern. Front Microbiol 12: 812591. Antilles N, Garc\u00eda-Bocanegra I, Alba-Casals A, L\u00f3pez-Soria S, P\u00e9rez-M\u00e9ndez N, Saco M (2021) Occurrence and Antimicrobial Resistance of Zoonotic Enteropathogens in Gulls from Southern Europe. Sci Total Environ 763: 143018. Du J, Luo J, Huang J, Wang C, Li M, Wang B, Chang H, Ji J, Sen K, He H (2019) Emergence of Genetic Diversity and Multi-Drug Resistant Campylobacter jejuni from Wild Birds in Beijing, China. Front Microbiol 10: 2433. Dudzic A, Urban-Chmiel R, St\u00eapie'n-Py'sniak D, Dec M, Puchalski A, Wernicki A (2016) Isolation, identification and antibiotic resistance of Campylobacter strains isolated from domestic and free-living pigeons. Br Poult Sci 57: 172-178. European Food Safety Authority (EFSA) and European Centre for Disease Prevention and Control (ECDC) (2023) The European Union One Health 2022 Zoonoses Report. EFSA Journal 21: e8442. Gabriele-Rivet V, Fairbrother JH, Tremblay D, Harel J, C\u00f4t\u00e9 N, Arsenault J (2016) Prevalence and risk factors for Campylobacter spp., Salmonella spp., Coxiella burnetii, and Newcastle disease virus in feral pigeons (Columba livia) in public areas of Montreal, Canada. Can J Res 80: 81-85. Khan IU, Hill S, Nowak E, Edge TA (2013) Effect of incubation temperature on the detection of thermophilic Campylobacter species from freshwater beaches, nearby wastewater effluents, and bird fecal droppings. Appl Environ Microbiol 79: 7639-7645. Lu J, Ryu H, Santo Domingo JW, Griffith JF, Ashbolt N (2011) Molecular detection of Campylobacter spp.in California gull (Larus californicus) excreta. Appl Environ Microbiol 77: 5034-5039. Luangtongkum T, Jeon B, Han J, Plummer P, Logue CM, Zhang Q (2009) Antibiotic resistance in Campylobacter: emergence, transmission and persistence. Future Microbiol 4: 189-200. Man SM (2011) The clinical importance of emerging Campylobacter species.&nbsp"},{"key":"#cr-split#-ref0.2","unstructured":"Nat Rev Gastroenterol Hepatol 8: 669-685. Menc\u00eda-Guti\u00e9rrez A, Garc\u00eda-Pe\u00f1a FJ, Gonz\u00e1lez F, Pastor-Tibur\u00f3n N, P\u00e9rez-Cobo I, Mar\u00edn M, Mart\u00edn-Maldonado B (2024) Exploring the Prevalence and Resistance of Campylobacter in Urban Bird Populations. Vet Sci 11: 210. Menc\u00eda-Guti\u00e9rrez A, Mart\u00edn-Maldonado B, Pastor-Tibur\u00f3n N, Moraleda V, Gonz\u00e1lez F, Garc\u00eda-Pe\u00f1a FJ (2021) Prevalence and Antimicrobial Resistance of Campylobacter from Wild Birds of Prey in Spain. Comp Immunol Microbiol Infect Dis 79: 101712. Obiri\u2010Danso K, Paul N, Jones K (2001) The effects of UVB and temperature on the survival of natural populations and pure cultures of Campylobacter jejuni, Camp. coli, Camp. lari and urease\u2010positive thermophilic campylobacters (UPTC) in surface waters. J Appl Microbiol 90: 256-267. Piddock LJ, Jin YF, Griggs DJ (2001) Effect of hydrophobicity and molecular mass on the accumulation of fluoroquinolones by Staphylococcus aureus. J Antimicrob Chemother 47: 261-270. Quinn PJ, Markey BK, Leonard FC, Fitzpatrick ES, Fanning S, Hartigan PJ (2011) Veterinary microbiology and microbial disease. 2nd ed., John Wiley and Sons, Chichester, UK, pp 287-292. Ramonait\u00eb S, Novoslavskij A, Zakarien\u00eb G, Aksomaitien\u00eb J, Malakauskas M (2015) High Prevalence and Genetic Diversity of Campylobacter jejuni in Wild Crows and Pigeons. Curr Microbiol 71: 559-565. Ramos R, Cerd\u00e0-Cu\u00e9llar M, Ram\u00edrez F, Jover L, Ruiz X (2010) Influence of refuse sites on the prevalence of Campylobacter spp. and Salmonella serovars in seagulls. Appl Environ Microbiol 76: 3052-3056. Russo TP, Pace A, Varriale L, Borrelli L, Gargiulo A, Pompameo M, Dipineto L (2021) Prevalence and Antimicrobial Resistance of Enteropathogenic Bacteria in Yellow-Legged Gulls (Larus Michahellis) in Southern Italy. Animals 11: 275. Waldenstrom J, Broman T, Carlsson I, Hasselquist D, Achterberg RP, Wagenaar JA, Olsen B (2002) Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds. Appl Environ Microbiol 68: 5911-5917. Whelan CJ, Wenny DG, Marquis RJ (2008) Ecosystem services provided by birds. Ann NY Acad Sci 1134: 25-60."}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"language":"pl","link":[{"URL":"https:\/\/journals.pan.pl\/Content\/138527\/5%20_%20S%C4%B1tk%C4%B1.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T13:27:28Z","timestamp":1773840448000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.pan.pl\/dlibra\/publication\/158500\/edition\/138527\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,17]]},"references-count":2,"URL":"https:\/\/doi.org\/10.24425\/pjvs.2026.158500","relation":{},"ISSN":["1505-1773"],"issn-type":[{"value":"1505-1773","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3,17]]}}}