{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T20:46:47Z","timestamp":1773866807280,"version":"3.50.1"},"reference-count":2,"publisher":"Polish Academy of Sciences Chancellery","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Canine adenovirus type 1 (CAdV-1), the causative agent of infectious canine hepatitis (ICH), a fatal disease affecting domestic and wild canids, yet its prevalence and molecular characteristics remain underexplored in India\u2019s north eastern region (NER). This study presents the first comprehensive genomic and immunoinformatic analysis of CAdV-1 in dogs in the region. Out of 208 canine parvovirus type-2 (CPV-2) positive fecal samples, 36 (17.30%) tested positive for CAdV-1 by PCR. Of the 36 positive samples, 25 samples were sequenced. Deduced amino acid analysis revealed notable amino acid mutations, including Asn127Asp, His129Arg, Trp148Ser, Leu201Pro, Thr206Met and Gly215Glu. Sequence analysis of the 25 field samples revealed distinct regional clustering consistent with regional viral evolution. In terms of relatedness to global strains, the NER isolates showed highest similarity to Asian and European canid and wildlife-origin isolates with 96% to 100% amino acid homology. Selection pressure analysis revealed predominantly purifying selection. aBSREL and Contrast-FEL identified a few codons potentially experiencing weak or episodic positive selection, likely reflecting host immune adaptation. GARD analysis ruled out evidence of recombination. Immunoinformatic prediction identified B-cell epitope, \u201cNKTTTEATIITY ISMTFLLVSLTLFLNLVTLTL,\u201d in most CAdV-1 sequences making it a suitable candidate for future vaccine development. The MHC-I binding peptide \u201cLTFPNVLITLNNKY\u201d (positions 83-96) demonstrated a strong affinity for the canine allele, suggesting its potential for triggering cytotoxic T-cell responses. These findings shed new light on the molecular epidemiology of CAdV-1 in the NER and highlight the critical need for multi-pathogen screening, molecular surveillance at wildlife \u2013 livestock interfaces, and future whole-genome studies to explicate viral evolution, host interactions, immune evasion, and regional strain diversity.<\/jats:p>","DOI":"10.24425\/pjvs.2026.158511","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T13:04:32Z","timestamp":1773839072000},"page":"101-101","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and immunoinformatic characterization of canine adenovirus type 1 in parvovirus-infected dogs: A comprehensive study from northeast India"],"prefix":"10.24425","author":[{"given":"P.K.","family":"Subudhi","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.N.","family":"Marak","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Maibam","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.B.","family":"Rajesh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lalhmangaihzuali","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.K.","family":"Behera","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Roychoudhury","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, West Bengal University of Animal & Fishery Sciences, Kolkata \u2013 700 037, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.K.","family":"Maibam","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Husbandry Services, Imphal, Manipur, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Jalukie, Nagaland, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.D.","family":"Sahu","sequence":"additional","affiliation":[{"name":"Office of Deputy Director of Veterinary Services Ambikapur, Surguja, Chhattisgarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry,Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Sahu","sequence":"additional","affiliation":[{"name":"Office of Deputy Director of Veterinary Services Ambikapur, Surguja, Chhattisgarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"37468","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"#cr-split#-ref0.1","unstructured":"Balboni A, Terrusi A, Urbani L, Troia R, Stefanelli SAM, Giunti M, Battilani M (2022) Canine circovirus and canine adenovirus type 1 and 2 in dogs with parvoviral enteritis. 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Mol Biol Evol 35: 773-777.","DOI":"10.1093\/nar\/gky1006"}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"language":"pl","link":[{"URL":"https:\/\/journals.pan.pl\/Content\/138533\/11%20_%20Behera.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T13:27:03Z","timestamp":1773840423000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.pan.pl\/dlibra\/publication\/158511\/edition\/138533\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,17]]},"references-count":2,"URL":"https:\/\/doi.org\/10.24425\/pjvs.2026.158511","relation":{},"ISSN":["1505-1773"],"issn-type":[{"value":"1505-1773","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3,17]]}}}