{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T17:42:40Z","timestamp":1748713360651,"version":"3.38.0"},"reference-count":0,"publisher":"American Veterinary Medical Association (AVMA)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[1992,4,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n<jats:p>Virulence factors were studied in 82 strains of <jats:italic>Escherichia coli<\/jats:italic> isolated from the urine of dogs with urinary tract infections. The most frequently expressed O antigens were 2, 4, 6, 25, and 22\/83. Most strains were K nontypeable. Mannose-sensitive hemagglutination (<jats:sc>msh<\/jats:sc>) with canine erythrocytes was observed in 71 strains and mannose-resistant hemagglutination (<jats:sc>mrh<\/jats:sc>) was observed in 32 strains. Strains that caused <jats:sc>msh<\/jats:sc> of erythrocytes from dogs also caused <jats:sc>msh<\/jats:sc> of erythrocytes from guinea pigs. Most strains that caused <jats:sc>mrh<\/jats:sc> of human A<jats:sub>1<\/jats:sub>p<jats:sub>1<\/jats:sub> erythrocytes also reacted with erythrocytes of dogs.<\/jats:p>\n<jats:p>Of 22 strains (27%)_that agglutinated human A<jats:sub>1<\/jats:sub>P<jats:sub>1<\/jats:sub> erythrocytes, but not A<jats:sub>1<\/jats:sub>P<jats:sub>1<\/jats:sub> erythrocytes, 17 (77%) had specificity for globo A, but did not react with the galactose \u03b11\u21924galactose\u03b2 disaccharide receptor. The remaining 5 strains and 2 others that simultaneously expressed an X adhesin agglutinated galactose \u03b11\u21924galactose\u03b2-coated latex beads.<\/jats:p>\n<jats:p>Bacterial adherence to canine uroepithelial cells from the bladder was most often observed in strains expressing <jats:sc>msh<\/jats:sc>, less often observed in strains expressing <jats:sc>mrh<\/jats:sc>, and least often observed in strains that failed to induce hemagglutination. Adherence of <jats:sc>msh<\/jats:sc> strains to canine uroepithelial cells was inhibited by \u03b1-methyl-<jats:sc>D<\/jats:sc>-mannoside. As a group, <jats:sc>mrh<\/jats:sc> strains expressing globo-A-and galactose \u03b11\u21924galactose\u03b2-specific adhesins did not have strong adherence.<\/jats:p>\n<jats:p>Strains of <jats:italic>E coli<\/jats:italic> isolated from dogs with urinaiy tract infections most commonly expressed type-1 fimbriae, and the main mechanism of in vitro adherence to canine uroepithelial cells involved a mannose-sensitive mechanism. Overrepresentation of globo-A-specific adhesins did not appear to be related to adherence of canine uroepithelial cells.<\/jats:p>","DOI":"10.2460\/ajvr.1991.53.04.494","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T16:24:16Z","timestamp":1706113456000},"page":"494-498","source":"Crossref","is-referenced-by-count":17,"title":["Serotype, hemolysin production, and adherence characteristics of strains of Escherichia coli causing urinary tract infection in dogs"],"prefix":"10.2460","volume":"53","author":[{"given":"David F.","family":"Senior","sequence":"first","affiliation":[{"name":"From the Department of Clinical Immunology, University of Gothenburg, Guldhedsgatan 10, S-413 46, Gothenburg, Sweden (Senior, deMan, Svanborg)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"deMan","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Immunology, University of Gothenburg, Guldhedsgatan 10, S-413 46, Gothenburg, Sweden (Senior, deMan, Svanborg)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catharina","family":"Svanborg","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Immunology, University of Gothenburg, Guldhedsgatan 10, S-413 46, Gothenburg, Sweden (Senior, deMan, Svanborg)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/53\/4\/ajvr.1991.53.04.494.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/53\/4\/ajvr.1991.53.04.494.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/53\/4\/ajvr.1991.53.04.494.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T14:53:58Z","timestamp":1741359238000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/53\/4\/ajvr.1991.53.04.494.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4,1]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.1991.53.04.494","relation":{},"ISSN":["0002-9645"],"issn-type":[{"type":"print","value":"0002-9645"}],"subject":[],"published":{"date-parts":[[1992,4,1]]}}}