{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:20:49Z","timestamp":1762100449249,"version":"3.38.0"},"reference-count":39,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine efficacy of an extracorporeal\ncircuit to maintain a segment of equine large colon\nfor 3.5 hours and to evaluate the effect of low arterial\nflow on histologic and metabolic variables.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Segments of large colon from\n15 healthy adult horses.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014The pelvic flexure was surgically\nremoved and maintained in an isolated circuit. In the\ncontrol group, tissue was evaluated for 3.5 hours,\nwhereas in the low-flow group, arterial flow was\nreduced to 20% of baseline for 40 minutes followed\nby 2 hours of reperfusion. Various metabolic and\nhemodynamic variables were evaluated at 30-minute\nintervals. Effects of nitric oxide (NO) and L-<jats:italic>N<\/jats:italic>-nitro-arginine-\nmethyl-ester (L-NAME) on contractile activity\nwere determined, and histomorphologic evaluation\nwas performed at the completion of the study.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Low-flow ischemia with reperfusion\ncaused significant histomorphologic differences,\ncompared with the control group. In the low-flow\ngroup, significant differences included reduction in\nPaCO<jats:sub>2<\/jats:sub>, reduction in bicarbonate concentrations,\nincrease in PaO<jats:sub>2<\/jats:sub>, and an increase in base deficit in\narterial and venous blood samples. Other significant\ndifferences included increases in PCV, protein concentration,\ntotal WBC count, and albumin clearance\nfor the low-flow group. Differences were not detected\nin inhibitory activity of the low-flow group relative\nto the control tissue with or without addition of NO\nand L-NAME.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusion<\/jats:bold>\u2014The extracorporeal circuit maintained a\nsegment of equine intestine for 3.5 hours and can be\nused to simulate ischemic injury. The extracorporeal\ncircuit provides the potential to investigate pharmaceutic\nagents that can minimize intestinal injury. 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