{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T21:09:32Z","timestamp":1775768972808,"version":"3.50.1"},"reference-count":43,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objectives<\/jats:bold>\n                    \u2014To determine whether samples of tracheal\nepithelial lining fluid (TELF) obtained from\nhorses have elastinolytic activity characteristic of\nmetalloproteinases, to compare elastinolytic activity\nin TELF obtained from healthy horses and horses\nwith chronic obstructive pulmonary disease\n(COPD), and to determine whether chemically modified\ntetracycline-3 (CMT-3) inhibits elastinolytic\nactivity in TELF.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Animals<\/jats:bold>\n                    \u201410 horses with COPD and 10 healthy control\nhorses.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Zymography and fluorometry were used\nto measure elastinolytic activity, and EDTA was used\nto inhibit elastinolytic activity and verify that the activity\nwas attributable to metalloproteinases. Possible\ninhibition of elastinolytic activity with CMT-3 was\nstudied in vitro.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014Elastinolytic activity was found in TELF\nobtained from all horses, and this activity was significantly\nhigher in TELF obtained from horses with\nCOPD than in TELF obtained from healthy horses. For\nall samples, EDTA and CMT-3 inhibited elastinolytic\nactivity.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\n                    \u2014Elastinolytic\nactivity is detectable in TELF obtained from horses\nand seems to be attributable to metalloproteinases.\nElastinolytic activity in TELF is significantly inhibited\nby CMT-3. Elastinolytic activity in TELF can be detected\nby means of zymography or fluorometry.\nIncreased elastinolytic activity may reflect destruction\nof pulmonary tissue in horses with COPD. Chemically\nmodified tetracyclines such as CMT-3 may provide an\nadditional treatment possibility for horses with COPD.\n(\n                    <jats:italic>Am J Vet Res<\/jats:italic>\n                    2000;61:1067\u20131073)\n                  <\/jats:p>","DOI":"10.2460\/ajvr.2000.61.1067","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T12:12:48Z","timestamp":1133871168000},"page":"1067-1073","source":"Crossref","is-referenced-by-count":13,"title":["Concentrations of elastinolytic metalloproteinases in respiratory tract secretions of healthy horses and horses with chronic obstructive pulmonary disease"],"prefix":"10.2460","volume":"61","author":[{"given":"Saara M.","family":"Raulo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo A.","family":"Sorsa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e4ivi 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