{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:58:01Z","timestamp":1761742681179,"version":"3.38.0"},"reference-count":26,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To characterize the effect that filtrate\nobtained from cultures of <jats:italic>Moraxella bovis<\/jats:italic> has on cultured\ncorneal epithelial cells and other types of cultured\nmammalian cells.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Cultured hamster corneal\nepithelial cells, bovine epithelial cells, and several\ntransformed cell lines exposed to culture filtrate from\na pathogenic isolate of <jats:italic>M bovis<\/jats:italic>.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014<jats:italic>Moraxella bovis<\/jats:italic> was cultured, and bacteria\nwere removed by filtration. The resulting bacterial\nculture filtrate was incubated with various types of\ncultured cells, and effects of the filtrate on detachment\nof various mammalian cell types was quantified\nby the use of neutral red dye. Additionally, bacterial\nculture filtrate was treated with protease inhibitors as\nwell as trypsin and heat prior to incubation with cultured\nmammalian cells.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Bacterial culture filtrate of <jats:italic>M bovis<\/jats:italic> caused\nall types of cultured cells to detach from each other\nand from the substrate, with the maximal effect evident\n2 hours after initiating incubation. Detached cells\nwere alive, and detachment was reversible. Serine\nprotease inhibitors (phenylmethylsulfonylfluoride and\n\u03b1<jats:sub>2<\/jats:sub>-macroglobulin) inhibited cell detachment attributable\nto bacterial culture filtrate. Heating and treatment\nwith trypsin also inhibited cell detachment.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014<jats:italic>Moraxella\nbovis<\/jats:italic> produces a soluble factor that causes reversible\ndetachment of cultured cells. This activity may play a\nrole in the pathogenesis of infectious bovine keratoconjunctivitis.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2000;61:1145\u20131149)<\/jats:p>","DOI":"10.2460\/ajvr.2000.61.1145","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T17:12:48Z","timestamp":1133889168000},"page":"1145-1149","source":"Crossref","is-referenced-by-count":8,"title":["Detection of cell detachment activity induced by Moraxella bovis"],"prefix":"10.2460","volume":"61","author":[{"given":"Ruth M.","family":"Marrion","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lela K.","family":"Riley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"p_1_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0007-1935(17)32882-8","volume":"135","author":"Baptista PJHP","year":"1979","journal-title":"Br Vet 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