{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:22:04Z","timestamp":1792725724558,"version":"4.5.0"},"reference-count":20,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine whether the tears of llamas,\nsheep, and cattle contain lysozyme and compare\nlysozyme concentrations in tears among these\nspecies.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201440 llamas, 5 sheep, and 36 cattle.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Electrophoresis, western blot immunoassay\nfor lysozyme, a spectrophotometric assay to\ndetect tear lysozyme by its ability to lyse a suspension\nof <jats:italic>Micrococcus lysodeiticus<\/jats:italic>, and a microtiter\nplate colorometric assay were performed.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014A 13.6-kd protein band was detected by\nuse of electrophoresis and western blot immunoassay\nin llama and sheep tears but not cattle tears.\nResults of spectrophotometric assay suggested that\nllama and sheep tears had high concentrations of\nlysozyme, whereas cattle tears had low concentrations.\nResults of the microtiter plate colorometric\nassay suggested that llama tears had high concentrations\nof lysozyme, whereas concentrations in sheep\nand cattle tears were lower.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Lysozyme\nconcentrations in tears may vary among species and\nthis variability may contribute to differing susceptibilities\nto ocular diseases such as infectious keratoconjunctivitis.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2000;61:1294\u20131297)<\/jats:p>","DOI":"10.2460\/ajvr.2000.61.1294","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T17:16:12Z","timestamp":1133889372000},"page":"1294-1297","source":"Crossref","is-referenced-by-count":12,"title":["Detection of lysozyme in llama, sheep, and cattle tears"],"prefix":"10.2460","volume":"61","author":[{"given":"Juliet R.","family":"Gionfriddo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harriet","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elikplimi K.","family":"Asem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheryl G.","family":"Krohne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"p_1_1","first-page":"1101","volume":"9","author":"Eichenbaum JD","year":"1987","journal-title":"Compend Contin Educ Pract Vet"},{"key":"p_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004397-197301310-00006"},{"key":"p_3_3","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1001\/archopht.1981.03930010300016","volume":"99","author":"Gillette TE","year":"1981","journal-title":"Arch Ophthalmol"},{"key":"p_4_4","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1111\/j.1755-3768.1984.tb08445.x","volume":"62","author":"Aine E","year":"1983","journal-title":"Acta Ophthalmol"},{"key":"p_5_5","unstructured":"Sherman MD, Cevallos V, Gabriel R, etal Measurement of human tear lysozyme using a novel synthetic substrate. 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