{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:19:08Z","timestamp":1762078748909,"version":"3.38.0"},"reference-count":33,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine patterns of methicillin-resistant\nstaphylococci isolated from apparently healthy\nhorses.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u201444 horses from 8 riding clubs\nin Japan.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Methicillin-resistant staphylococci were\nisolated from the skin or nares, using a selective\nmedium containing a \u03b2-(symboric) lactam antibiotic,\nceftizoxime. Clonality of isolates was determined by\nuse of pulsed-field gel electrophoresis. Detection of\n<jats:italic>mecA<\/jats:italic>, <jats:italic>mecI<\/jats:italic>, and <jats:italic>mecR1<\/jats:italic> genes was accomplished by\nuse of polymerase chain reactions.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Result<\/jats:bold>\u2014Of the 44 horses, 13 (29.5%) yielded 15 isolates\nof methicillin-resistant staphylococci. The 15 isolates\nwere identified as 6 species (<jats:italic>Staphylococcus epidermidis,\nS lentus, S saprophyticus, S xylosus, S sciuri,<\/jats:italic>\nand <jats:italic>S haemolyticus<\/jats:italic>). However, methicillin-resistant <jats:italic>S\naureus<\/jats:italic> was seldom isolated. Each isolate contained\nthe <jats:italic>mecA<\/jats:italic> gene and had a high resistance to \u03b2- lactam\nantibiotics. Some isolates also were resistant to other\nantibiotics such as erythromycin and kanamycin.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Methicillinresistant\ncoagulase-negative staphylococci that were\nhighly resistant to various antibiotics were isolated\nfrom apparently healthy horses in Japan. These organisms\nmust be considered a potential threat to horses\nand veterinarians who care for them. 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