{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T04:04:48Z","timestamp":1758945888544,"version":"3.38.0"},"reference-count":41,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine whether platelet-activating\nfactor (PAF) is involved in acute lung microvascular\ninjury associated with pneumonic pasteurellosis in\ncalves.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201415 healthy 2- to 4-week-old male Holstein\ncalves.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Calves were anesthetized and inoculated\nintrabronchially with saline (0.9% NaCl) solution\n(n = 5) or 1 \u00d7 10<jats:sup>9<\/jats:sup>\n\t\t\t\t\t<jats:italic>Pasteurella haemolytica<\/jats:italic> organisms\n(n = 10). Of the 10 calves inoculated with <jats:italic>P haemolytica<\/jats:italic>,\n5 also were treated with WEB 2086, a potent\ninhibitor of PAF, and 5 were treated with vehicle.\nBlood and bronchoalveolar lavage samples were collected\nbefore and 1, 2, 4, and 6 hours after inoculation\nof <jats:italic>P haemolytica<\/jats:italic>. Blood samples were analyzed to\nevaluate total number and differential counts of leukocytes,\ndilute whole-blood leukocyte deformability,\nsize of neutrophils, and neutrophil CD11b expression.\nBronchoalveolar lavage samples were analyzed for\ntotal number and differential counts of nucleated\ncells, total protein concentration, and hemoglobin\nconcentration. Size and gross and histologic appearance\nof lung lesions also was determined.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Treatment of calves with WEB 2086\nreduced size of lung lesions, attenuated the increase\nin microvascular permeability, and reduced neutrophil\ninfiltration in the first 4 hours after inoculation.\nTreatment with WEB 2086 also attenuated a\ndecrease in leukocyte deformability, increase in size\nof neutrophils, and CD11b expression by circulating\nneutrophils.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014It appears that\nPAF is a major mediator for altered lung microvascular\npermeability and activation of circulating neutrophils in\nthe first 4 hours after onset of pneumonic pasteurellosis\nin calves. 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