{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:51:57Z","timestamp":1787320317946,"version":"build-2736575974"},"reference-count":35,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To evaluate a rapid polymerase chain\nreaction (PCR) fingerprinting technique for discriminating\namong\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    isolates from laboratory\nrabbits.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Sample Population<\/jats:bold>\n                    \u201433\n                    <jats:italic>P multocida<\/jats:italic>\n                    isolates from\nrabbits with clinical pasteurellosis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014PCR assays were conducted with 2\nminisatellites (core sequence and modified core\nsequence of phage M13) and 2 microsatellites\n([GTG]\n                    <jats:sub>5<\/jats:sub>\n                    and [GACA]\n                    <jats:sub>4<\/jats:sub>\n                    ). Each bacterium was assigned\nto a PCR type for each of the primers used. Boiled\nbacterial extracts and purified genomic DNA were\ncompared by use of PCR assays for phage M13 and\n(GACA)\n                    <jats:sub>4<\/jats:sub>\n                    . Plasmids were isolated from each bacterium,\nand their influence on PCR fingerprint was determined,\nusing boiled extracts as a DNA source.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014M13 core sequence and M13 modified\ncore sequence yielded 5 and 8 PCR types, respectively.\nThe microsatellites (GTG)\n                    <jats:sub>5<\/jats:sub>\n                    and (GACA)\n                    <jats:sub>4<\/jats:sub>\n                    yielded\n4 and 9 PCR fingerprint types, respectively.\nFingerprint patterns obtained by use of isolated DNA\ndiffered from those obtained by use of boiled\nextracts, although discrimination among\n                    <jats:italic>P multocida<\/jats:italic>\n                    isolates was similar. The presence or absence of plasmids\ndid not affect PCR fingerprints.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    \u2014Single primer PCR fingerprinting with\nminisatellite and microsatellite primers is an efficient\nand reproducible method for the discrimination of\n                    <jats:italic>P multocida<\/jats:italic>\n                    isolates from rabbits and can be performed\ndirectly, using boiled bacterial extracts as a source of\ntemplate, although more bands were obtained from\npure genomic DNA. 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