{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T06:52:22Z","timestamp":1760079142166,"version":"3.38.0"},"reference-count":47,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine the capacity of inflammatory\nmediators tumor necrosis factor-\u03b1 (TNF-\u03b1), interleukin-\n8 (IL-8), platelet-activating factor (PAF),\nlipopolysaccharide (LPS), and leukotoxin to prime, activate,\nor alter deformability of adult bovine neutrophils.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Blood collected from 5 healthy\nadult Holstein cows.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Isolated neutrophils or whole blood was\nincubated with TNF-\u03b1, IL-8, PAF, LPS, or leukotoxin,\nand neutrophil chemiluminescence, degranulation,\ndeformability, shape change, CD11b expression, and\nsize distribution was measured.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Incubation with TNF-\u03b1, IL-8, PAF, and LPS\nprimed neutrophils for oxygen radical release but\ncaused minimal oxygen radical release by themselves.\nNone of the inflammatory mediators induced\ndegranulation. Incubation with TNF-\u03b1 and PAF resulted\nin a decrease in neutrophil deformability and\ninduced shape change in neutrophils. Incubation with\nPAF consistently resulted in an increase in neutrophil\nsize as measured by use of flow cytometry. Only IL-8\ncaused an increase in expression of CD11b by neutrophils.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Inflammatory\nmediators tested had minimal effects on neutrophil\noxygen radical production or degranulation but did\nprime neutrophils for oxygen radical production.\nIncubation with PAF and TNF-\u03b1 caused a decrease in\nneutrophil deformability and altered neutrophil shape\nand size. Results of our study indicate that PAF- and\nTNF-\u03b1-induced changes in neutrophil deformability\nand size may cause integrin- and selectin-independent\ntrapping of neutrophils in the lungs of cattle with\npneumonic pasteurellosis. 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