{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T21:09:23Z","timestamp":1775768963319,"version":"3.50.1"},"reference-count":42,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To measure concentrations of nitric oxide\nmetabolites (nitrite-nitrate [NOt]) in cartilage, synovial\nmembrane, and cranial cruciate ligament (CCL) in\ndogs and evaluate associations with osteoarthritis in\ndogs with CCL rupture.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Animals<\/jats:bold>\n                    \u201446 dogs with CCL rupture and 54 control\ndogs without joint disease.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Tissue specimens for histologic examination\nand explant culture were harvested during surgery\nin the CCL group or immediately after euthanasia in the\ncontrol group; NOt concentrations were measured in\nsupernatant of explant cultures and compared among\ndogs with various degrees of osteoarthritis and\nbetween dogs with and without CCL rupture.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014Osteoarthritic cartilage had significantly\nhigher NOt concentration (1,171.6 nmol\/g) than did\nhealthy cartilage (491.0 nmol\/g); NOt concentration\nwas associated with severity of macroscopic and\nmicroscopic lesions. Synovial membrane NOt concentration\ndid not differ between dogs with and without\nCCL rupture. Ruptured CCL produced less NOt\nthan did intact ligaments. In control dogs, NOt concentrations\nwere similar for intact ligaments\n(568.1 nmol\/g) and articular cartilage (491.0 nmol\/g).\nSynthesis of NOt was inhibited substantially by coincubation\nwith inhibitors.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\n                    \u2014Results suggest\nthat NOt in canine joint tissues originates from\nthe inducible nitric oxide synthase pathway. Nitric\noxide metabolite production in cartilage was greater\nin dogs with osteoarthritis than in healthy dogs and\nwas associated with lesion severity, suggesting that\nnitric oxide inhibitors may be considered as a treatment\nfor osteoarthritis. The CCL produces substantial\nconcentrations of NOt; the importance of this finding\nis unknown. 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