{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T22:54:50Z","timestamp":1777330490342,"version":"3.51.4"},"reference-count":23,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To assess whether dogs with blastomycosis\nproduce antibodies against the WI-1 and A-antigens\nof <jats:italic>Blastomyces dermatitidis<\/jats:italic> and whether the\nantibodies are useful in serodiagnosis.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014359 serum samples obtained\nfrom 245 dogs.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014233 samples from 122 dogs with blastomycosis,\nand 1 sample each from 24 dogs with suspected\nblastomycosis, 51 control dogs without infection,\nand 48 healthy dogs from an enzootic region\nwere obtained. Antibodies against WI-1 antigen were\ndetected by radioimmunoassay (RIA). Serum samples\nwere tested in parallel for antibodies against the Aantigen\nof <jats:italic>B dermatitidis<\/jats:italic> by commercial agar-gel\nimmunodiffusion (AGID) in a reference laboratory.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Antibodies were detected in 92% of infected\ndogs by RIA and in 41% by AGID. For 29 serum\nsamples that were obtained 11 to 1,545 days after\ndiagnosis, antibodies were detected in 92% of samples\nby RIA and 7% by AGID. For 93 serial serum\nsamples from 29 dogs with blastomycosis, the mean\nanti-WI-1 titer was 1:18,761 at the time of diagnosis,\nand decreased to a mean of 1:1,338 by 210 days after\ntreatment was initiated. Of 24 dogs with suspected\ninfection, antibodies were detected in 67% by RIA\nand 33% by AGID. Control dogs without blastomycosis\nhad no detectable antibodies in either assay. Thus,\nsensitivity was 92% for RIA and 41% for AGID, and\nspecificity was 100% for both tests.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Anti-WI-1\nantibodies are readily detected by RIA in dogs with\nblastomycosis. Titers become high, decline during\ntreatment, and persist for months. Anti-A antibodies\nare sometimes detected with AGID, but these\ndecrease quickly. 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