{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T05:21:46Z","timestamp":1741411306053,"version":"3.38.0"},"reference-count":24,"publisher":"American Veterinary Medical Association (AVMA)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To evaluate the activity of Kupffer cells\n(KC) of control neonatal pigs and neonatal pigs treated\nwith endotoxin and to compare activity of KC with\nthat of pulmonary alveolar macrophages (PAM).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Kupffer cells and PAM\nobtained from 24 neonatal pigs (7 to 10 days old).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Pairs (n = 7) of littermates served as\ntreated (lipopolysaccharide [LPS]) or untreated pigs.\nPigs were euthanatized 24 hours after treatment, and\ncells were isolated. Cells were obtained from 10\nother neonatal pigs for other assays. Functional activity\nof cells was evaluated by use of in vitro assays to\nevaluate bactericidal activity, phagocytosis, and production\nof superoxide anion (SOA), nitric oxide (NO),\nand tumor necrosis factor-\u03b1 (TNF-\u03b1). Each assay was\nrepeated on cells obtained from 4 to 6 pigs.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Phagocytic activity was similar in KC and\nPAM, but bactericidal activity and production of SOA\nand TNF-\u03b1 was lower in KC. Neither KC nor PAM produced\nNO in response to LPS stimulation.\nPhagocytosis, bactericidal activity, and production of\nSOA were enhanced for KC obtained from neonatal\npigs treated with LPS. The PAM from LPS-treated\nneonatal pigs had similar bactericidal activity to PAM\nobtained from untreated pigs.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Functional\ncapacity of KC is affected by endotoxin. This provides\nadditional information of the role the liver plays in\nimmune surveillance. In addition, the response of KC\nin neonatal pigs exposed to endotoxin is of value for\nunderstanding gram-negative bacterial sepsis, which\nis a major cause of mortality in neonatal pigs. (<jats:italic>Am J\nVet Res<\/jats:italic> 2001;62:1040\u20131045)<\/jats:p>","DOI":"10.2460\/ajvr.2001.62.1040","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T17:27:58Z","timestamp":1133890078000},"page":"1040-1045","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of phagocytosis, bactericidal activity, and production of superoxide anion, nitric oxide, and tumor necrosis factor-\u03b1 in Kupffer cells of neonatal pigs"],"prefix":"10.2460","volume":"62","author":[{"given":"J. Kinyamu","family":"Akunda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin A.","family":"Ahrens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodore T.","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"p_1_243","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1001\/archsurg.1988.01400350114018","volume":"123","author":"West MA","year":"1988","journal-title":"Arch Surg"},{"key":"p_2_244","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb19222.x"},{"key":"p_3_245","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1002\/jlb.55.6.723","volume":"55","author":"Limuro Y","year":"1994","journal-title":"J Leukoc Biol"},{"key":"p_4_246","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1164\/ajrccm\/146.1.224","volume":"146","author":"Decamp MM","year":"1992","journal-title":"Am Rev Respir Dis"},{"key":"p_5_247","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.2527\/jas1985.6161576x","volume":"61","author":"Caperna TJ","year":"1985","journal-title":"J Anim Sci"},{"key":"p_6_248","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(87)90457-6"},{"key":"p_7_249","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(91)90121-U"},{"key":"p_8_250","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1007\/BF02631264","volume":"30","author":"Gerlach JC","year":"1994","journal-title":"In Vitro Cell Dev Biol Anim"},{"key":"p_9_251","first-page":"G213","volume":"267","author":"Olynk JK","year":"1994","journal-title":"Am J Physiol"},{"key":"p_10_252","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0165-2427(91)90042-B","volume":"28","author":"Stevens MG","year":"1991","journal-title":"Vet Immunol Immunopathol"},{"key":"p_11_253","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(89)90148-7"},{"key":"p_12_254","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(81)90138-1"},{"key":"p_13_255","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(97)00078-0"},{"key":"p_14_256","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1995.1163"},{"key":"p_15_257","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/jlb.50.4.364","volume":"50","author":"Chitko-McKown CG","year":"1991","journal-title":"J Leukoc Biol"},{"key":"p_16_258","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.4049\/jimmunol.155.5.2595","volume":"155","author":"Dieter P","year":"1995","journal-title":"J Immunol"},{"key":"p_17_259","first-page":"G920","volume":"271","author":"Lichtman SN","year":"1996","journal-title":"Am J Physiol"},{"key":"p_18_260","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1128\/IAI.63.5.1863-1869.1995","volume":"63","author":"Fahmi H","year":"1995","journal-title":"Infect Immun"},{"key":"p_19_261","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1995.1098"},{"key":"p_20_262","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1097\/00000658-199404000-00010","volume":"219","author":"Roland CR","year":"1994","journal-title":"Ann Surg"},{"key":"p_21_263","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(97)00139-6"},{"key":"p_22_264","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1128\/IAI.63.5.2075-2078.1995","volume":"63","author":"Michaliszyn E","year":"1994","journal-title":"Infect Immun"},{"key":"p_23_265","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(96)05748-0"},{"key":"p_24_266","first-page":"G891","volume":"261","author":"Bautista AP","year":"1991","journal-title":"Am J Physiol"}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/62\/7\/ajvr.2001.62.1040.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/62\/7\/ajvr.2001.62.1040.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/62\/7\/ajvr.2001.62.1040.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T12:02:40Z","timestamp":1741348960000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/62\/7\/ajvr.2001.62.1040.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7,1]]},"references-count":24,"journal-issue":{"issue":"7"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.2001.62.1040","relation":{},"ISSN":["0002-9645"],"issn-type":[{"type":"print","value":"0002-9645"}],"subject":[],"published":{"date-parts":[[2001,7,1]]}}}