{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:45:46Z","timestamp":1790873146277,"version":"4.1.0"},"reference-count":27,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2001,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine the virulence of a <jats:italic>Brucella\nabortus<\/jats:italic> mutant, BA25, lacking a major 25 kd outer\nmembrane protein ( <jats:italic>Omp25<\/jats:italic>) in cattle.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201420 mixed-breed heifers in late gestation.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u201410 heifers were inoculated with 1 \u00d7 10<jats:sup>7<\/jats:sup>\ncolony-forming units of the <jats:italic>Omp25<\/jats:italic> mutant via the\nconjunctival sac, and an equal number were infected\nwith the virulent parental strain <jats:italic>B abortus<\/jats:italic> 2308. The\ndelivery status of the dams was recorded, and colonization\nwas assessed following necropsy. The ability\nof BA25 to replicate inside bovine phagocytes and\nchorionic trophoblasts was also evaluated in vitro\nbecause of the propensity of virulent brucellae to\nreplicate inside these cells in vivo.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014The parental strain induced abortions in 5 of\n10 inoculated cattle, whereas only 1 of 10 dams\nexposed to BA25 aborted. <jats:italic>Brucella abortus<\/jats:italic> strain\n2308 colonized all of the cow-calf pairs and induced\n<jats:italic>Brucella<\/jats:italic>-specific antibodies in 100% of the dams. In\ncontrast, BA25 was isolated by bacteriologic cultural\ntechnique from 30% of the calves and 50% of the\ninoculated dams (n = 10). Of the 10 heifers inoculated\nwith BA25, 4 did not develop <jats:italic>Brucella<\/jats:italic>-specific antibodies\nnor were they colonized by the mutant strain.\nIn bovine macrophages and chorionic trophoblasts,\nBA25 replicated in significantly lower numbers than\nthe virulent parental strain (n = 3).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014The 25 kd outer\nmembrane protein may be an important virulence factor\nfor <jats:italic>B abortus<\/jats:italic> in cattle. The attenuation of the <jats:italic>Omp25<\/jats:italic>\nmutant in cattle may involve the inability of BA25 to replicate\nefficiently in bovine phagocytes and chorionic trophoblasts.(<jats:italic>Am J Vet Res<\/jats:italic> 2001;62:1461\u20131466)<\/jats:p>","DOI":"10.2460\/ajvr.2001.62.1461","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T17:20:00Z","timestamp":1133889600000},"page":"1461-1466","source":"Crossref","is-referenced-by-count":50,"title":["Attenuation of a Brucella abortus mutant lacking a major 25 kDa outer membrane protein in cattle"],"prefix":"10.2460","volume":"62","author":[{"given":"Matthew D.","family":"Edmonds","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natha J.","family":"Booth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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