{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T04:49:26Z","timestamp":1778647766147,"version":"3.51.4"},"reference-count":40,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2001,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To investigate the effects of enrofloxacin\nand magnesium deficiency on explants of equine\narticular cartilage.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Articular cartilage explants and\ncultured chondrocytes obtained from adult and\nneonatal horses.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Full-thickness explants and cultured\nchondrocytes were incubated in complete or magnesium-\ndeficient media containing enrofloxacin at concentrations\nof 0, 1, 5, 25, 100, and 500 \u00b5g\/ml.\nIncorporation and release of sulfate <jats:sup>35<\/jats:sup>S over 24 hours\nwere used to assess glycosaminoglycan (GAG) synthesis\nand degradation. An assay that measured binding\nof dimethylmethylene blue dye was used to compare\ntotal GAG content between groups. Northern\nblots of RNA from cultured chondrocytes were\nprobed with equine cDNA of aggrecan, type-II collagen,\nbiglycan, decorin, link protein, matrix metalloproteinases\n1, 3, and 13, and tissue inhibitor of metalloproteinase\n1.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014A dose-dependent suppression of <jats:sup>35<\/jats:sup>S incorporation\nwas observed. In cartilage of neonates, <jats:sup>35<\/jats:sup>S\nincorporation was substantially decreased at\nenrofloxacin concentrations of 25 mg\/ml. In cartilage\nof adult horses, <jats:sup>35<\/jats:sup>S incorporation was decreased only\nat enrofloxacin concentrations of \u2265 100 \u00b5g\/ml.\nMagnesium deficiency caused suppression of <jats:sup>35<\/jats:sup>S\nincorporation. Enrofloxacin or magnesium deficiency\ndid not affect GAG degradation or endogenous GAG\ncontent. Specific effects of enrofloxacin on steadystate\nmRNA for the various genes were not\nobserved.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusion and Clinical Relevance<\/jats:bold>\u2014Enrofloxacin\nmay have a detrimental effect on cartilage metabolism\nin horses, especially in neonates. (<jats:italic>Am J Vet Res<\/jats:italic>\n2001;62:160\u2013166)<\/jats:p>","DOI":"10.2460\/ajvr.2001.62.160","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T14:33:45Z","timestamp":1135175625000},"page":"160-166","source":"Crossref","is-referenced-by-count":41,"title":["Effects of enrofloxacin and magnesium deficiency on matrix metabolism in equine articular cartilage"],"prefix":"10.2460","volume":"62","author":[{"given":"Celia L. 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