{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:11:37Z","timestamp":1777615897291,"version":"3.51.4"},"reference-count":56,"publisher":"American Veterinary Medical Association (AVMA)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2001,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine effects of the selectin\ninhibitor TBC1269 on neutrophil-mediated pulmonary\ndamage during acute <jats:italic>Mannheimia haemolytica-induced<\/jats:italic> pneumonia\nin newborn calves.\n        <\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u2014Eighteen 1- to 3-day-old colostrumdeprived\ncalves.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014<jats:italic>Mannheimia haemolytica<\/jats:italic> or saline\n(0.9% NaCl) solution was inoculated in both cranial\nlung lobes of 12 and 6 calves, respectively. Calves\nwere euthanatized 2 (saline, n = 3; <jats:italic>M haemolytica<\/jats:italic>,\nn = 4) or 6 hours (saline, n = 3; <jats:italic>M haemolytica<\/jats:italic>, n = 8)\nafter inoculation. Four <jats:italic>M haemolytica<\/jats:italic>-inoculated\ncalves euthanatized at 6 hours also received TBC1269\n(25 mg\/kg, IV) 30 minutes before and 2 hours after\ninoculation. Conjugated diene (CD) concentrations,\ninducible nitric oxide synthase (iNOS) expression, and\napoptotic cell counts were determined in lung specimens\ncollected during necropsy.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Conjugated diene concentrations were significantly\nincreased in all <jats:italic>M haemolytica<\/jats:italic>-inoculated groups,\ncompared with saline-inoculated groups. Calves treated\nwith TBC1269 had decreased concentrations of CD,\ncompared with untreated calves, although the difference\nwas not significant. Number of apoptotic neutrophils and\nmacrophages increased significantly in TBC1269-treated\ncalves, compared with untreated calves. Inducible nitric\noxide synthase was expressed by epithelial cells and\nleukocytes. However, iNOS was less abundant in airway\nepithelial cells associated with inflammatory exudates.\nDegree of iNOS expression was similar between\nTBC1269-treated and untreated calves.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions<\/jats:bold>\u2014<jats:italic>Mannheimia haemolytica<\/jats:italic> infection in\nneonatal calves resulted in pulmonary tissue damage\nand decreased epithelial cell iNOS expression. The\nselectin inhibitor TCB1269 altered, but did not completely\ninhibit, neutrophil-mediated pulmonary damage.\n( <jats:italic>Am J Vet Res<\/jats:italic> 2001;62:17\u201322)<\/jats:p>","DOI":"10.2460\/ajvr.2001.62.17","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T17:19:10Z","timestamp":1133889550000},"page":"17-22","source":"Crossref","is-referenced-by-count":19,"title":["Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves"],"prefix":"10.2460","volume":"62","author":[{"given":"Zaher A.","family":"Radi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica M.","family":"Caverly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Dixon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim A.","family":"Brogden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark R.","family":"Ackermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"p_1_50","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1002\/jlb.56.6.672","volume":"56","author":"Smith J.","year":"1994","journal-title":"J Leukoc Biol"},{"key":"p_2_51","first-page":"28","volume":"273","author":"Collins T","year":"1995","journal-title":"Sci Am"},{"key":"p_3_52","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.120.5.1227"},{"key":"p_4_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jlb.63.1.1","volume":"63","author":"Crockett-Torabi E","year":"1998","journal-title":"J Leukoc Biol"},{"key":"p_5_54","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.4.895"},{"key":"p_6_55","first-page":"2253","volume":"46","author":"Slocombe RF","year":"1985","journal-title":"Am J Vet Res"},{"key":"p_7_56","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.2460\/ajvr.1997.58.11.1227","volume":"58","author":"Saban R","year":"1997","journal-title":"Am J Vet Res"},{"key":"p_8_57","first-page":"746","volume":"101","author":"Sugino K","year":"1987","journal-title":"Surgery"},{"key":"p_9_58","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1998.0236"},{"key":"p_10_59","first-page":"L242","volume":"267","author":"Haddad IY","year":"1994","journal-title":"Am J Physiol"},{"key":"p_11_60","doi-asserted-by":"crossref","first-page":"337","DOI":"10.2460\/ajvr.1991.52.02.337","volume":"52","author":"Weiss DJ","year":"1991","journal-title":"Am J Vet Res"},{"key":"p_12_61","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1996.0034"},{"key":"p_13_62","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1093\/intimm\/7.7.1107","volume":"7","author":"Mulligan MS","year":"1995","journal-title":"Int Immunol"},{"key":"p_14_63","doi-asserted-by":"publisher","DOI":"10.1038\/364149a0"},{"key":"p_15_64","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1001\/archsurg.1995.01430110057011","volume":"130","author":"Ridings PC","year":"1995","journal-title":"Arch Surg"},{"key":"p_16_65","unstructured":"Kogan TP, Dupre B, Scott IL, etal, inventors; Texas Biotechnology Corporation, assignee. Di- and trivalent small molecule selectin inhibitors. US patent WO 9701335. January 16, 1997."},{"key":"p_17_66","doi-asserted-by":"publisher","DOI":"10.1021\/jm9704917"},{"key":"p_18_67","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1164\/ajrccm.159.4.9806002","volume":"159","author":"Abraham WM","year":"1999","journal-title":"Am J Respir Crit Care Med"},{"key":"p_19_68","doi-asserted-by":"crossref","first-page":"3595","DOI":"10.1128\/IAI.63.9.3595-3599.1995","volume":"63","author":"Brogden KA","year":"1995","journal-title":"Infect Immun"},{"key":"p_20_69","first-page":"800","volume":"19","author":"Tornusciolo DR","year":"1995","journal-title":"Biotechniques"},{"key":"p_21_70","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/0007-1935(90)90031-W","volume":"146","author":"Chen W","year":"1990","journal-title":"Br Vet J"},{"key":"p_22_71","doi-asserted-by":"crossref","first-page":"C1424","DOI":"10.1152\/ajpcell.1996.271.5.C1424","volume":"271","author":"Beckman JS","year":"1996","journal-title":"Am J Physiol"},{"key":"p_23_72","doi-asserted-by":"crossref","first-page":"5279","DOI":"10.1128\/IAI.65.12.5279-5288.1997","volume":"65","author":"Jungi TW","year":"1997","journal-title":"Infect Immun"},{"key":"p_24_73","unstructured":"SAS user's guide: statistics. Version 6. Cary, NC: SAS Institute Inc, 1990."},{"key":"p_25_74","unstructured":"Frank GH. Pasteurellosis in cattle. In: Adlam C, Rutter JM, eds. Pasteurella and pasteurellosis. San Diego: Academic Press, 1989;197-223."},{"key":"p_26_75","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(88)90114-1"},{"key":"p_27_76","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1152\/jappl.1987.63.2.840","volume":"63","author":"Brigham KL","year":"1987","journal-title":"J Appl Physiol"},{"key":"p_28_77","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1111\/j.1348-0421.1981.tb00026.x","volume":"25","author":"Sakaguchi S","year":"1981","journal-title":"Microbiol Immunol"},{"key":"p_29_78","doi-asserted-by":"publisher","DOI":"10.1016\/0748-5514(85)90130-8"},{"key":"p_30_79","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1097\/00003246-198608000-00010","volume":"14","author":"Takeda K","year":"1986","journal-title":"Crit Care Med"},{"key":"p_31_80","doi-asserted-by":"crossref","first-page":"2094","DOI":"10.1152\/jappl.1986.60.6.2094","volume":"60","author":"Demling RH","year":"1986","journal-title":"J Appl Physiol"},{"key":"p_32_81","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1177\/030098589603300602","volume":"33","author":"Ackermann MR","year":"1996","journal-title":"Vet Pathol"},{"key":"p_33_82","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1182\/blood.V84.3.889.889","volume":"84","author":"Fortenberry JD","year":"1994","journal-title":"Blood"},{"key":"p_34_83","doi-asserted-by":"crossref","first-page":"37","DOI":"10.2460\/ajvr.1998.59.01.37","volume":"59","author":"Lee EK","year":"1998","journal-title":"Am J Vet Res"},{"key":"p_35_84","doi-asserted-by":"crossref","first-page":"3177","DOI":"10.4049\/jimmunol.153.7.3177","volume":"153","author":"Burns AR","year":"1994","journal-title":"J Immunol"},{"key":"p_36_85","first-page":"152","volume":"47","author":"Hauser MA","year":"1986","journal-title":"Am J Vet Res"},{"key":"p_37_86","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(86)80761-2"},{"key":"p_38_87","doi-asserted-by":"publisher","DOI":"10.1007\/BF00924792"},{"key":"p_39_88","doi-asserted-by":"publisher","DOI":"10.1016\/0195-6701(88)90206-X"},{"key":"p_40_89","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1203\/00006450-198710000-00001","volume":"22","author":"Hill HR.","year":"1987","journal-title":"Pediatr Res"},{"key":"p_41_90","doi-asserted-by":"crossref","first-page":"452","DOI":"10.2460\/ajvr.1999.60.04.452","volume":"60","author":"Inanami O","year":"1999","journal-title":"Am J Vet Res"},{"key":"p_42_91","first-page":"893","volume":"78","author":"Granville DJ","year":"1998","journal-title":"Lab Invest"},{"key":"p_43_92","doi-asserted-by":"crossref","first-page":"5124","DOI":"10.4049\/jimmunol.150.11.5124","volume":"150","author":"Whyte MK","year":"1993","journal-title":"J Immunol"},{"key":"p_44_93","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1172\/JCI113970","volume":"83","author":"Savill JS","year":"1989","journal-title":"J Clin Invest"},{"key":"p_45_94","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199312303292706"},{"key":"p_46_95","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90267-4"},{"key":"p_47_96","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1002\/jlb.56.6.759","volume":"56","author":"Wizemann TM","year":"1994","journal-title":"J Leukoc Biol"},{"key":"p_48_97","doi-asserted-by":"crossref","first-page":"11419","DOI":"10.1073\/pnas.90.23.11419","volume":"90","author":"Charles IG","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"p_49_98","doi-asserted-by":"crossref","first-page":"7809","DOI":"10.1073\/pnas.92.17.7809","volume":"92","author":"Guo FH","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"p_50_99","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1073\/pnas.91.21.10089","volume":"91","author":"Asano K","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"p_51_100","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1073\/pnas.91.6.2046","volume":"91","author":"Vane JR","year":"1994","journal-title":"Proc Natl Acad Sci USA"},{"key":"p_52_101","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1172\/JCI119121","volume":"99","author":"Wheeler MA","year":"1997","journal-title":"J Clin Invest"},{"key":"p_53_102","doi-asserted-by":"crossref","first-page":"426","DOI":"10.2460\/ajvr.1999.60.04.426","volume":"60","author":"Hammond RA","year":"1999","journal-title":"Am J Vet Res"},{"key":"p_54_103","doi-asserted-by":"crossref","first-page":"7738","DOI":"10.1073\/pnas.82.22.7738","volume":"82","author":"Stuehr DJ","year":"1985","journal-title":"Proc Natl Acad Sci USA"},{"key":"p_55_104","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.1096\/fasebj.6.12.1381691","volume":"6","author":"Nathan C","year":"1992","journal-title":"FASEB J"},{"key":"p_56_105","doi-asserted-by":"publisher","DOI":"10.1006\/pupt.1999.0173"}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/62\/1\/ajvr.2001.62.17.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/62\/1\/ajvr.2001.62.17.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/62\/1\/ajvr.2001.62.17.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T12:55:47Z","timestamp":1741352147000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/62\/1\/ajvr.2001.62.17.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1,1]]},"references-count":56,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.2001.62.17","relation":{},"ISSN":["0002-9645"],"issn-type":[{"value":"0002-9645","type":"print"}],"subject":[],"published":{"date-parts":[[2001,1,1]]}}}