{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T03:11:19Z","timestamp":1768878679653,"version":"3.49.0"},"reference-count":42,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine efficacy of intranasal vaccination\nof rabbits with <jats:italic>Pasteurella multocida<\/jats:italic> A:3 outer\nmembrane proteins (OMP) expressing iron-regulated\nOMP (IROMP) in conferring protection against experimental\nchallenge exposure.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201452 male New Zealand White rabbits.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014 Rabbits were vaccinated intranasally on\ndays 0, 7, and 14; some vaccines included cholera\ntoxin (CT) as an adjuvant. Concentrations of intranasal\nIgA and serum IgG antibodies against <jats:italic>P multocida<\/jats:italic>\nOMP were determined. In experiment A, rabbits\nwere vaccinated with either phospate-buffered saline\nsolution (PBSS), PBSS-CT, OMP-CT, or IROMP-CT,\nchallenge-exposed intranasally on day 16, and euthanatized\nand necropsied on day 28. Rabbits were also\nvaccinated with OMP or IROMP without CT and were\nnot challenge-exposed. In experiment B, rabbits were\nvaccinated with PBSS, PBSS-CT, IROMP, or IROMP-CT.\nOn day 17, rabbits were challenge-exposed\nintranasally .Nasal bacteria and antibodies were determined\non day 24.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014In experiment A, OMP-CT vaccination stimulated\nmucosal and systemic antibody responses to\nthe bacterium and enhanced resistance against challenge\nexposure. Intranasal bacterial counts were not\nsignificantly reduced. Vaccination with IROMP-CT\nstimulated mucosal and systemic antibodies,\nenhanced resistance to challenge exposure, and significantly\nreduced nasal bacterial counts. In experiment\nB, natural infection was detected in several rabbits\nat challenge exposure; however, IROMP-CT-vaccinated\nrabbits had significantly higher serum and nasal\nantibody responses, compared with other\nrabbitsIROMP-CT-vaccinated rabbits had significantly\nlower nasal bacterial counts compared to control rabbits.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Intranasal\nvaccination of rabbits with <jats:italic>P multocida<\/jats:italic> outer membranes\ncontaining IROMP and CT stimulated immunity\nagainst experimental pneumonic pasteurellosis.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2001;62:697\u2013703)<\/jats:p>","DOI":"10.2460\/ajvr.2001.62.697","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T17:28:14Z","timestamp":1133890094000},"page":"697-703","source":"Crossref","is-referenced-by-count":16,"title":["Intranasal vaccination of rabbits with Pasteurella multocida A:3 outer membranes that express iron-regulated proteins"],"prefix":"10.2460","volume":"62","author":[{"given":"Anthony W.","family":"Confer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Suckow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Montelongo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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