{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T05:23:17Z","timestamp":1741411397319,"version":"3.38.0"},"reference-count":28,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To develop and validate an ex vivo model\nfor study of adherence of <jats:italic>Mannheimia haemolytica<\/jats:italic>\n(formerly <jats:italic>Pasteurella haemolytica<\/jats:italic>) to respiratory tract\nmucosa of cattle and to use this model to confirm\nadherence of <jats:italic>M haemolytica<\/jats:italic> serovar 1 (Mh1) to several\nrelevant respiratory mucosal surfaces.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Excised nasal, nasopharyngeal,\nturbinate, and tonsillar mucosal tissue from the\nbovine upper respiratory tract.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Mh1 was radiolabeled by use of tritiated\nleucine. Various concentrations of labeled bacteria\nwere incubated with bovine upper respiratory tract\ntissues for various times. Tissue was washed to\nremove nonadherent bacteria, and percentage of bacteria\nadhered (percentage of adherence) was estimated\nusing radioactivity. Using an optimal inoculum\nconcentration and incubation time, percentage of\nMh1 adherence was compared on nasal, nasopharyngeal,\nturbinate, and tonsillar mucosal tissue, and\nadherence to nasopharyngeal tissue was confirmed\nby scanning and transmission electron microscopy.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014The optimal Mh1 inoculum concentration\nwas 1 \u00d7 10<jats:sup>7<\/jats:sup> colony forming units\/ml and incubation\ntime was 3 hours. Percentage of adherence of Mh1\nto nasopharyngeal tissue was greater than adherence\nto other tissue types.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014The ex vivo\nmodel maintained the functional and structural integrity\nof bovine upper respiratory tract mucosa, as confirmed\nby light and electron microscopy. Electron\nmicroscopy revealed participation of epithelial cell cilia\nand surface mucus in adherence of Mh1 to nasopharyngeal\ntissue. Adherence of Mh1 was confirmed in\nrepeated assays, indicating that this organism\nadheres to upper respiratory tract mucosa of cattle.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2001;62:805\u2013811)<\/jats:p>","DOI":"10.2460\/ajvr.2001.62.805","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T17:28:14Z","timestamp":1133890094000},"page":"805-811","source":"Crossref","is-referenced-by-count":7,"title":["Development of an ex vivo model to study adherence of Mannheimia haemolytica serovar 1 to mucosal tissues of the respiratory tract of cattle"],"prefix":"10.2460","volume":"62","author":[{"given":"Jean M.","family":"Clarke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca J.","family":"Morton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyril 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