{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:32:29Z","timestamp":1789900349923,"version":"4.0.1"},"reference-count":31,"publisher":"American Veterinary Medical Association (AVMA)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine the susceptibility of ducks\nto avian pneumovirus (APV) of turkey origin.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201430 Pekin ducks that were 2 weeks old.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Ducks were assigned to 3 groups (10\nducks\/group). Ducks of groups 1 and 2 were inoculated\n(day 0) with 200 \u00b5l of cell-culture fluid containing\nAPV of turkey origin (10<jats:sup>5.5<\/jats:sup> median tissue-culture infective\ndose\/ml) by the oculonasal (group 1) or oral\n(group 2) route. Ducks of group 3 served as noninoculated\ncontrol birds. Two ducks from each group were\neuthanatized 3, 6, 9, 15, and 21 days after inoculation.\nBlood samples, tissue samples from the lungs, trachea,\nnasal turbinates, duodenum, diverticulum\nvitellinum (Meckel's diverticulum), and cecum, and\nswab specimens from the choana, cloaca, and trachea\nwere obtained from all birds during necropsy\nand examined for APV by use of reverse transcriptase-\npolymerase chain reaction (RT-PCR), virus isolation,\nand histologic examination. Blood samples also\nwere examined for APV antibodies, using an ELISA.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Tissue samples obtained up to 21 days after\ninoculation had positive results when tested by use of\nRT-PCR. Virus was isolated from nasal turbinates of\nbirds inoculated via the oculonasal route. Serum samples\nobtained 15 and 21 days after inoculation had\npositive results when tested for APV-specific antibody.\nClinical signs of disease were not observed in ducks\ninoculated with APV of turkey origin.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Ducks inoculated\nwith APV of turkey origin may not develop clinical\nsigns of disease, but they are suspected to play a\nrole as nonclinical carriers of APV. 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