{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T19:21:03Z","timestamp":1780428063964,"version":"3.54.1"},"reference-count":23,"publisher":"American Veterinary Medical Association (AVMA)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2002,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To measure and compare the concentration\nand composition of very low-density lipoprotein\n(VLDL) in plasma and selected lipids in serum of horses\nfed mixed grass hay ad libitum or denied feed for\n36 hours.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u20144 healthy adult mares.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Mares were either fed mixed grass hay\nad libitum or denied feed for 36 hours beginning at\n8:00 AM. Blood samples were collected every 2 hours\nduring the study period and analyzed for nonesterified\nfatty acid (NEFA), triglyceride (TG), VLDL, and glucose\nconcentrations and composition of VLDL.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Withholding feed significantly increased\nmean serum concentrations of NEFA. By 36 hours, a\n16-fold increase in mean serum NEFA concentration\nand 2-fold increase in mean plasma VLDL concentration,\ncompared with baseline values, were detected.\nMean plasma TG concentrations significantly\nincreased with time in feed-deprived horses.\nSignificantly lower overall mean plasma glucose concentrations\nwere detected in feed-deprived horses.\nMean percentage of protein in VLDL was significantly\nlower in feed-deprived horses. Plasma VLDL concentrations\nvaried widely among horses in response\nto withholding feed. Plasma TG and VLDL concentrations\nremained unaltered in 2 horses.<\/jats:p>\n\t\t\t\t<jats:p>C<jats:bold>onclusions and Clinical Relevance<\/jats:bold>\u2014Withholding\nfeed significantly increases blood lipid concentrations\nin horses, but individual horses respond differently.\nSerum NEFA concentrations were increased in all 4\nhorses denied feed, indicating mobilization of tissue\ntriglyceride stores. Variation in plasma VLDL concentration\nin response to withholding feed suggests that\nits metabolism is strongly influenced by other, as yet\nundetermined, factors in horses. Differences in the\nplasma VLDL concentrations among horses in\nresponse to withholding feed may be used as an indication\nof susceptibility to the hyperlipemic syndrome\nof Equidae. 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