{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:26:44Z","timestamp":1790008004846,"version":"4.0.1"},"reference-count":32,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2002,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To evaluate splenic mast cell tumors\n(MCT) of cats for activating mutations in the protooncogene\nc-\n                    <jats:italic>kit<\/jats:italic>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Sample Population<\/jats:bold>\n                    \u201410 formalin-fixed, paraffinembedded\nsplenic MCT from cats in the pathology\ndatabase of the Veterinary Medical Teaching Hospital\nat the University of California, Davis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Genomic DNA was isolated from tumor\nspecimens, and the polymerase chain reaction (PCR)\nprocedure was performed for exons 11, 12, and 17.\nThe PCR products were analyzed by use of agarose\ngel electrophoresis and then directly sequenced.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014We did not identify mutations in the juxtamembrane\ndomain (encoded by exons 11 and 12) or\ncatalytic domain (encoded by exon 17) of c-\n                    <jats:italic>kit<\/jats:italic>\n                    in any\nof the splenic MCT specimens.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\n                    \u2014Although\nmutations in the proto-oncogene c-\n                    <jats:italic>kit<\/jats:italic>\n                    occur frequently\nin naturally developing MCT in dogs and aggressive\nmastocytosis in humans, the data reported here documented\nthat dysregulation of Kit function through\nactivating mutations is unlikely in splenic MCT of cats.\nTherapeutic strategies aimed at inhibiting Kit signaling\n(ie, kinase inhibitors such as imatinib [STI571]) may\nnot be of benefit for the treatment of this disease in\ncats. (\n                    <jats:italic>Am J Vet Res<\/jats:italic>\n                    2002;63:1129\u20131133).\n                  <\/jats:p>","DOI":"10.2460\/ajvr.2002.63.1129","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T09:53:55Z","timestamp":1135158835000},"page":"1129-1133","source":"Crossref","is-referenced-by-count":27,"title":["Activating mutations in the catalytic or juxtamembrane domain of c- kit in splenic mast cell tumors of cats"],"prefix":"10.2460","volume":"63","author":[{"given":"Gillians","family":"Dank","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"May B.","family":"Chien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl 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