{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:51:32Z","timestamp":1770979892726,"version":"3.50.1"},"reference-count":10,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To develop polymerase chain reaction-restriction\nfragment length polymorphism (PCR-RFLP)\nanalysis for molecular typing of strains of\n<jats:italic>Streptococcus zooepidemicus<\/jats:italic> and to use the new\ntyping method to analyze a collection of isolates from\nthe respiratory tract of Thoroughbreds.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u201410 strains of <jats:italic>S zooepidemicus<\/jats:italic>,\n65 isolates from the respiratory tract of 9 yearlings\nfollowing long distance transportation, and 89 isolates\nfrom tracheal aspirates of 20 foals with pneumonia.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Phenotypic variations in the <jats:italic>SzP<\/jats:italic> protein\nwere detected by western immunoblot analysis.\nUsing PCR-RFLP analysis, genotypes were obtained\nwith primer sets from the <jats:italic>SzP<\/jats:italic> gene, followed by\nrestriction endonuclease digestion of the amplicons.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Unique genotypic patterns were obtained\nwith a primer set designed from both ends of the\nstructural gene and the restriction endonuclease <jats:italic>Dde<\/jats:italic>\nI. Forty-five isolates from the lymphoid tissue within\nthe pharyngeal recess (ie, pharyngeal tonsil) of yearlings\nincluded 10 <jats:italic>SzP<\/jats:italic> genotypes and <jats:italic>SzP<\/jats:italic> phenotypes.\nIsolates from the trachea of each yearling were of a\nsingle genotype that was also present among isolates\nfrom the pharyngeal tonsil of the same horses.\nIsolates from tracheal aspirates of foals belonged to\n14 genotypes.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusion and Clinical Relevance<\/jats:bold>\u2014Analysis of the\n<jats:italic>SzP<\/jats:italic> gene by use of PCR-RFLP was effective for molecular\ntyping of strains of <jats:italic>S zooepidemicus<\/jats:italic> in the study of\nrespiratory tract disease in horses. Results of PCR-RFLP\nanalysis indicate that a single strain of <jats:italic>S zooepidemicus<\/jats:italic>\ncan migrate from the pharyngeal tonsil to the trachea at\na high rate in horses undergoing long distance transportation.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2002;63:1298\u20131301)<\/jats:p>","DOI":"10.2460\/ajvr.2002.63.1298","type":"journal-article","created":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T18:27:15Z","timestamp":1138040835000},"page":"1298-1301","source":"Crossref","is-referenced-by-count":18,"title":["Polymerase chain reaction-restriction fragment length polymorphism analysis of the SzP gene of Streptococcus zooepidemicus isolated from the respiratory tract of horses"],"prefix":"10.2460","volume":"63","author":[{"given":"Toru","family":"Anzai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Timoney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Kuwamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichi","family":"Wada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Msaaki","family":"Oikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Higuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"p_1_374","doi-asserted-by":"crossref","unstructured":"Hayakawa Y, Komae H, Ide H, etalAn occurrence of equine transport pneumonia caused by mixed infection with Pasteurella caballi. Streptococcus suis and Streptococcus zooepidemicus J Vet Med Sci 1993;55:455-456.","DOI":"10.1292\/jvms.55.455"},{"key":"p_2_375","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0021-9975(05)80066-0","volume":"113","author":"Oikawa M","year":"1995","journal-title":"J Comp Pathol"},{"key":"p_3_376","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.2460\/ajvr.1993.54.10.1615","volume":"54","author":"Hoffman AM","year":"1993","journal-title":"Am J Vet Res"},{"key":"p_4_377","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.2460\/ajvr.1993.54.10.1602","volume":"54","author":"Hoffman MA","year":"1993","journal-title":"Am J Vet Res"},{"key":"p_5_378","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2000.61.162"},{"key":"p_6_379","first-page":"416","volume":"155","author":"Moore BO","year":"1969","journal-title":"J Am Vet Med Assoc"},{"key":"p_7_380","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.2460\/ajvr.1998.59.09.1129","volume":"59","author":"Walker JA","year":"1998","journal-title":"Am J Vet Res"},{"key":"p_8_381","doi-asserted-by":"crossref","first-page":"4126","DOI":"10.1128\/JCM.38.11.4126-4130.2000","volume":"38","author":"Nicholson ML","year":"2000","journal-title":"J Clin Microbiol"},{"key":"p_9_382","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1128\/IAI.63.4.1440-1445.1995","volume":"63","author":"Timoney JF","year":"1995","journal-title":"Infect Immun"},{"key":"p_10_383","unstructured":"Timoney JF, Mukhtar M. 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Equine infectious disease, in Proceedings. 6th Int Conf Cambridge 1993;15-20."}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/63\/9\/ajvr.2002.63.1298.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/63\/9\/ajvr.2002.63.1298.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/63\/9\/ajvr.2002.63.1298.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T12:04:42Z","timestamp":1741349082000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/63\/9\/ajvr.2002.63.1298.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9,1]]},"references-count":10,"journal-issue":{"issue":"9"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.2002.63.1298","relation":{},"ISSN":["0002-9645"],"issn-type":[{"value":"0002-9645","type":"print"}],"subject":[],"published":{"date-parts":[[2002,9,1]]}}}