{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:09:18Z","timestamp":1790143758170,"version":"4.0.1"},"reference-count":37,"publisher":"American Veterinary Medical Association (AVMA)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2002,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To establish an immortalized cell line and\ntransplantable xenograft of feline bronchioloalveolar\nlung carcinoma (BAC).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Pleural effusion from a 12-yearold\nPersian male cat with BAC.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Tumor cells from the pleural effusion\nwere grown in monolayer cell culture and injected\ninto severe combined immunodeficient (SCID) mice\nto establish an immortalized cell line as well as a\ntransplantable xenograft.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Both the primary lung carcinoma, the\nderived cell line, and the transplantable xenograft had\nevidence of a type-II pneumocyte origin expressing\nlamellar bodies ultrastructurally and thyroid transcription\nfactor-1 and surfactant immunocytochemically. All\n3 also expressed nuclear p53 immunoreactivity. A\nmetaphase spread of the cell line (SPARKY) probed\nwith fluorescein-labeled genomic feline DNA gave evidence\nof its feline origin. Flow cytometric studies indicated\naneuploidy with a DNA index of 1.6. An R-banded\nkaryotype revealed a modal number of 66 including\nthe feline Y chromosome. The cell line had a doubling\ntime of 16 hours. The xenograft (SPARKY-X)\nreached a diameter of 1 cm in 3 weeks in SCID mice.\nDeoxyribonucleic acid fingerprint analysis revealed\nthat SPARKY and SPARKY-X were novel and strongly\nmatched each other, except for the murine component\nfound in SPARKY-X. Interestingly, SPARKY-X\nmanifested the characteristic lepidic growth pattern of\npulmonic BAC.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions<\/jats:bold>\u2014Both the cell line and xenograft\nretained their autochthonous BAC phenotype, making\nthem useful for the subsequent dissection of molecular\nabnormalities in feline BAC and in vitro screening\nof chemotherapeutic agents. 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