{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T21:10:29Z","timestamp":1775769029157,"version":"3.50.1"},"reference-count":36,"publisher":"American Veterinary Medical Association (AVMA)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To evaluate lactoferrin and lysozyme content\nin various ocular glands of bison and cattle and in\ntears of bison.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Sample Population<\/jats:bold>\n                    \u2014Tissues of ocular glands\nobtained from 15 bison and 15 cattle and tears collected\nfrom 38 bison.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Immunohistochemical analysis was\nused to detect lysozyme and lactoferrin in formalin-fixed,\nparaffin-embedded sections of the ocular\nglands. Protein gel electrophoresis was used to analyze\nocular glands and pooled bison tears by use of a\ntris-glycine gel and SDS-PAGE. Western blotting was\nused to detect lactoferrin and lysozyme.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014Immunohistochemical staining for lactoferrin\nwas evident in the lacrimal gland and gland of the third\neyelid in cattle and bison and the deep gland of the third\neyelid (Harder's gland) in cattle. Equivocal staining for\nlactoferrin was seen for the Harder's gland in bison. An\n80-kd band (lactoferrin) was detected via electrophoresis\nand western blots in the lacrimal gland and gland of\nthe third eyelid in cattle and bison, Harder's glands of\ncattle, and bison tears. An inconsistent band was seen\nin Harder's glands of bison. Lysozyme was not detected\nin the lacrimal gland of cattle or bison with the use of\nimmunohistochemical analysis or western blots.\nWestern blots of bison tears did not reveal lysozyme.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion and Clinical Relevance<\/jats:bold>\n                    \u2014Distribution\nof lactoferrin and a lack of lysozyme are similar in the\nlacrimal gland of cattle and bison. Differences in other\ntear components may be responsible for variability in\nthe susceptibility to infectious corneal diseases that\nexists between bison and cattle. 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